XTT Cell Proliferation Assay Kit
Cayman Chemical Item Number 10010200
XTT Cell Proliferation Assay Kit
Description
Defining the mechanisms responsible for alterations in cell cycle progression is crucial to understanding many human diseases, most notably cancer. Cell proliferation assays have been widely used to assess cell cycle regulatory factors such as growth factors, cytokines, mitogens, and drugs.1 Cayman’s XTT cell proliferation assay kit provides an easy to use tool for studying induction and inhibition of cell proliferation in any in vitro model. The assay is based on the extracellular reduction of XTT by NADH produced in the mitochondria via trans-plasma membrane electron transport and an electron mediator.2 Reduction of XTT produces a water-soluble formazan which dissolves directly into the culture medium, eliminating the need for an additional solubilization step. This kit will allow investigators to screen drug candidates involved in cell cycle regulation.
1
Francoeur, A., and Assalian, A. Microcat: A novel cell proliferation and cytotoxicity assay based on WST-1. Biochemica 3 19-25 (1996).
2
Berridge, M.V., Tan, A.S., and Herst, P.M. Tetrazolium dyes as tools in cell biology: New insights into their cellular reduction. Biotechnol Annu Rev 11 127-152 (2005).
| Stability |
1 year |
| Storage |
-20°C |
| Shipping |
Wet ice
in continental US; may vary elsewhere
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Background Reading
ulic, S., Panic, L., Ðikic, I., et al. Deregulation of cell growth and malignant transformation. Croat Med J 46(4) 622-638 (2005).
Berridge, M.V., Tan, A.S., and Herst, P.M. Tetrazolium dyes as tools in cell biology: New insights into their cellular reduction. Biotechnol Annu Rev 11 127-152 (2005).
Francoeur, A., and Assalian, A. Microcat: A novel cell proliferation and cytotoxicity assay based on WST-1. Biochemica 3 19-25 (1996).
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Pricing updated 2012-02-12.
Prices are subject to change without notice.
To ask for assistance with one of our products please contact a Technical Support Scientist.
Warning This product is not for human or veterinary use.
You may be eligible to receive a free sample XTT Cell Proliferation Assay Kit under the Cayman Challenge program.
Let Cayman analyze your samples for you. See EIA Service for details and availability.
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Cayman Chemical is a manufacturer, supplier and vendor of biochemical reagents, assay kits, antibodies, and proteins.
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