Cayman Chemical | 1,8-ANS | Description • 1-Anilinonaphthalene-8-Sulfonic Acid (CAS 82-76-8)

10010219 1,8-ANS

1-Anilinonaphthalene-8-Sulfonic Acid (CAS 82-76-8)

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1,8-ANS is a fluorescent dye that binds with high affinity to hydrophobic surfaces of proteins. The emission maximum of 1,8-ANS undergoes a blue shift and fluorescence intensity increases significantly upon binding to low polarity regions of a protein surface.^1,2 These properties make 1,8-ANS well suited for determining the affinity of hydrophobic ligands to their corresponding binding proteins, such as the binding of free fatty acids to fatty acid binding proteins (FABPs).^1,2,3 1,8-ANS binds to intestinal FABP (FABP2) with a K_d value of ~9.7 µM at 24.5°C.²

1 Pastukhov, A.V., Ropson, I.J. Fluorescent dyes as probes to study lipid-binding proteins. Proteins: Structure, Function, and Genetics 53, 607-615 (2003).

2 Kirk, W.R., Kurian, E., Prendergast, F.G. Characterization of the sources of protein-ligand affinity: 1-sulfonato-8-(1')anilinonaphthalene binding to intestinal fatty acid binding protein. Biophys J 70 69-83 (1996).

3 Ory, J.J., Banaszak, L.J. Studies of the ligand binding reaction of adipocyte lipid binding protein using the fluorescent probe 1,8-anilinonaphthalene-8-sulfonate. Biophys J 77 1107-1116 (1999).

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Pricing updated 2010-03-20.
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