See personalized New Product recommendations! Get personalized New Product recommendations! Register or Login for personalized New Product recommendations!

Join us! · InformexUSA 2012 · New Orleans, Louisiana · February 14-17, 2012 · Booth 2514

1,8-ANS

Cayman Chemical Item Number 10010219

1-Anilinonaphthalene-8-Sulfonic Acid (CAS 82-76-8)

1,8-ANS (CAS 82-76-8)

See more

Description

1,8-ANS is a fluorescent dye that binds with high affinity to hydrophobic surfaces of proteins. The emission maximum of 1,8-ANS undergoes a blue shift and fluorescence intensity increases significantly upon binding to low polarity regions of a protein surface.1,2 These properties make 1,8-ANS well suited for determining the affinity of hydrophobic ligands to their corresponding binding proteins, such as the binding of free fatty acids to fatty acid binding proteins (FABPs).1,2,3 1,8-ANS binds to intestinal FABP (FABP2) with a Kd value of ~9.7 µM at 24.5°C.2

1 Pastukhov, A.V., and Ropson, I.J. Fluorescent dyes as probes to study lipid-binding proteins. Proteins 53 607-615 (2003).

2 Kirk, W.R., Kurian, E., and Prendergast, F.G. Characterization of the sources of protein-ligand affinity: 1-sulfonato-8-(1')anilinonaphthalene binding to intestinal fatty acid binding protein. Biophys J 70 69-83 (1996).

3 Ory, J.J., and Banaszak, L.J. Studies of the ligand binding reaction of adipocyte lipid binding protein using the fluorescent probe 1,8-anilinonaphthalene-8-sulfonate. Biophys J 77 1107-1116 (1999).

Synonyms
  • 1-Anilinonaphthalene-8-Sulfonic Acid
Formal Name 8-​(phenylamino)-​1-​naphthalenesulfonic acid
CAS Number 82-76-8
Molecular Formula C16H13NO3S
Formula Weight 299.3
Formulation A crystalline solid
Purity >98%
λmax 219, 270, 374 nm
Stability 2 years
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere
SMILES
Copy SMILES to clipboard
OS(=O)​(=O)​c1cccc2cccc(Nc3ccccc3)​c12

Background Reading

Pastukhov, A.V., and Ropson, I.J. Fluorescent dyes as probes to study lipid-binding proteins. Proteins 53 607-615 (2003).

Ory, J.J., and Banaszak, L.J. Studies of the ligand binding reaction of adipocyte lipid binding protein using the fluorescent probe 1,8-anilinonaphthalene-8-sulfonate. Biophys J 77 1107-1116 (1999).

Kirk, W.R., Kurian, E., and Prendergast, F.G. Characterization of the sources of protein-ligand affinity: 1-sulfonato-8-(1')anilinonaphthalene binding to intestinal fatty acid binding protein. Biophys J 70 69-83 (1996).
Size Price Quantity Subtotal
5 g $15.00 $0.00
10 g $29.00 $0.00
50 g $120.00 $0.00
100 g $210.00 $0.00
Bulk Contact
Cart Total $0.00

This product is available in custom sizes and/or larger quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-02-12. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

Related Products

FABP1 (human recombinant) Exclusive
FABP1 (rat recombinant)
FABP2 (rat recombinant)
FABP3 (human recombinant)
FABP4 (human recombinant)
FABP4 (human recombinant) Western Ready Control
FABP4 (mouse recombinant) Western Ready Control
FABP5 (human recombinant)
FABP7 (human recombinant)
Show all 9 Hide all but first 3

Downloads

Batch-specific Information

Login to access batch-specific information

Cayman Chemical is a manufacturer, supplier and vendor of biochemical reagents, assay kits, antibodies, and proteins.

Other Resources
Management Team Company Profile Company History ChemAssistant Tools FAQs Cayman Europe Cayman Pharma CaBRI Cayman Gear Press Releases Illustrations and Charts Key Research Area Posters Article Library Analysis Tools Conference Schedule Order Terms Browser Recommendation Privacy Statement Site Map

Cayman Chemical Company · 1180 East Ellsworth Road · Ann Arbor, Michigan 48108 · USA

Toll Free: (800) 364-9897 (USA and Canada Only) · Fax: (734) 971-3640

Copyright 2012 Cayman Chemical Company

Lost password?