FABP4 Inhibitor/Ligand Screening Assay Kit

Cayman Chemical Item Number 10010231

aP2; Adipocyte FABP; A-FABP; Fatty Acid Binding Protein 4

Description

Fatty acid binding protein 4 (Adipocyte-FABP, aP2, FABP4) is one of nine known cytosolic fatty acid binding proteins ranging in size from 14-15 kDa containing 127-132 amino acids.¹ FABP4 is highly expressed in adipocytes and is regulated by peroxisome-proliferator-activated receptor-γ (PPARγ) agonists, insulin and fatty acids. Recent studies using FABP4 gene deletion in mice indicate a dominant role for FABP4 in several chronic metabolic diseases. Therefore, inhibiting the function of FABP4 is a potential mechanism for the treatment of metabolic diseases like diabetes and atherosclerosis.^2,3 Cayman’s FABP4 Ligand Binding Assay Kit provides a simple, reproducible, and sensitive tool for the identification of FABP4 ligands. The assay makes use of a Detection Reagent that exhibits increased fluorescence at 500 nm when bound to FABP4. Any strong ligand and/or inhibitor of FABP4 will displace the Detection Reagent thereby reducing the fluorescence. FABP4 is provided in high purity and in sufficient quantity for 100 tests.

1 Zimmerman, A.W., and Veerkamp, J.H. New insights into the structure and function of fatty acid-binding proteins. Cell Mol Life Sci 59 1096-1116 (2002).

2 Furuhashi, M., Tuncman, G., Görgün, C.Z., et al. Treatment of diabetes and atherosclerosis by inhibiting fatty-acid-binding protein aP2. Nature 447 959-965 (2007).

3 Boord, J.B., Maeda, K., Makowski, L., et al. Adipocyte fatty acid-binding protein, aP2, alters late atherosclerotic lesion formation in severe hypercholesterolemia. Arterioscler Thromb Vasc Biol 22 1686-1691 (2002).

Synonyms

aP2
Adipocyte FABP
A-FABP
Fatty Acid Binding Protein 4

Stability

1 year

Storage

-80°C

Shipping

Dry ice in continental US; may vary elsewhere

Background Reading

Zimmerman, A.W., and Veerkamp, J.H. New insights into the structure and function of fatty acid-binding proteins. Cell Mol Life Sci 59 1096-1116 (2002).

Furuhashi, M., Tuncman, G., Görgün, C.Z., et al. Treatment of diabetes and atherosclerosis by inhibiting fatty-acid-binding protein aP2. Nature 447 959-965 (2007).

Boord, J.B., Maeda, K., Makowski, L., et al. Adipocyte fatty acid-binding protein, aP2, alters late atherosclerotic lesion formation in severe hypercholesterolemia. Arterioscler Thromb Vasc Biol 22 1686-1691 (2002).

Massolini, G., and Calleri, E. Survey of binding properties of fatty acid-binding proteins chromatographic methods. J Chromatogr B 797 255-268 (2003).

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10010231-1EA

FABP Assay Buffer (10X)

1 ea

FABP4 (human recombinant) Assay Reagent (5X)

1 ea

FABP Assay Detection Reagent (5X)

1 ea

Arachidonic Acid Control

1 ea

96-Well Solid Plate (black)