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10010365 8-Isoprostane Affinity Sorbent
8-iso Prostaglandin F2α; 8-epi Prostaglandin F2α
8-Isoprostane Affinity Sorbent Workbook Links
Cayman offers a variety of analysis tools to assist you.
Chemical structure definitions are available for many Cayman products. See Chemical Structure Database for details.
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Traditional purification of urine and plasma samples prior to measurement by EIA has required the use of reverse phase C-18 columns and various organic solvents. As this method relies upon polarity for separation, the final purified product is a non-specific mixture of many compounds. Further purification by TLC or HPLC is then required to isolate a single product. As an alternative, affinity chromatography provides a specific, reliable, and convenient means of purification for a variety of sample types. Cayman Chemical has developed an affinity sorbent for the purification of 8-isoprostane (8-iso PGF2α) from urine and plasma samples. The affinity sorbent can be regenerated by following a simple washing procedure, depending on the samples, please see product insert for details. Each vial of sorbent contains 1 ml of 8-isoprostane affinity sorbent (mouse anti-8-isoprostane covalently bound to Sepharose 4B). 8-Isoprostane is a potent pulmonary and renal vasoconstrictor1 and has been implicated as a causative mediator of hepatorenal syndrome and pulmonary oxygen toxicity.2 It has also been proposed as a marker of antioxidant activity. Cayman Chemical’s 8-Isoprostane EIA Kit (Catalog Nos. 10367 and 10368) is a highly sensitive and accurate assay, and our affinity purification reagents improve it even more.
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1
Banerjee, M., Kang, K.H., Morrow, J.D., et al. Effects of a novel prostaglandin, 8-epi-PGF2α, in rabbit lung in situ. Am J Physiol 263 H660-H663 (1992).
2
Vacchiano, C.A., Tempel, G.E. Role of nonenzymatically generated prostanoid, 8-iso-PGF2α, in pulmonary oxygen toxicity. J Appl Physiol 77 2912-2917 (1994).
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Purchase 10010365 8-Isoprostane Affinity Sorbent
Pricing is for North America only. Other customers should contact a distributor in their region.
This product is also available to buy in bulk quantities.
Please contact our
Sales Department
for a quote or to purchase.
Cayman strives to be a reliable biochemical reagent vendor by providing the best possible products and services.
To ask for assistance with one of our products please contact our Technical Help staff.
Warning This product is not for human or veterinary use.
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Pricing updated 2010-03-19.
Prices are subject to change without notice.
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