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Join us! · InformexUSA 2012 · New Orleans, Louisiana · February 14-17, 2012 · Booth 2514

p38 MAPK (Phospho-Thr180/Tyr182) Cell-Based Phosphorylation/Translocation Assay Kit

Cayman Chemical Item Number 10010374

p38 MAPK (Phospho-Thr180/Tyr182) Cell-Based Phosphorylation/Translocation Assay Kit

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Description

p38 MAPK is activated by phosphorylation at Thr180 and Tyr182 in response to both inflammatory cytokines and stress, making it an important enzyme in diseases like asthma and autoimmunity, as well as in the stress response of the nervous system. The subcellular location of p38 following stimulation is not well understood. Some evidence suggests that activated p38 translocates from the cytoplasm to the nucleus, whereas other reports indicate that phosphorylated p38 is present in the cytoplasm of stimulated cells. Cayman’s p38 Phosphorylation/Translocation Cell-Based Assay Kit provides a highly specific phospho-p38 MAPK (phospho-Thr180 and Tyr182) primary antibody together with a Dylight™ (product of Pierce Biotechnology, Inc.) conjugated secondary antibody in a ready-to-use format. Thrombin, which has been shown by scientists at Cayman Chemical to cause the translocation of phospho-p38 MAPK (phospho-Thr180 and Tyr182) into nuclei, is included as a positive control.
Stability 1 year
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere

Background Reading

Pearson, G., Robinson, F., Gibson, B.T., et al. Mitogen-activated protein (MAP) kinase pathways: Regulation and physiological functions. Endocr Rev 22(2) 153-183 (2001).

Shi, Y., and Gaestel, M. In the cellular garden of forking paths: How p38 MAPKs signal for downstream assistance.. Biol Chem 383 1519-1536 (2002).

Philip, S., and Armstead, W.M. Differential role of PTK, ERK, and p38 MAPK in superoxide impairment of NMDA cerebrovasodilation.. Brain Res 979 98-103 (2003).

Roux, P.P., and Blenis, J. ERK and p38 MAPK-activated protein kinases: A family of protein kinases with diverse biological functions. Microbiol Mol Biol Rev 68(2) 320-344 (2004).

Hu, M.C.T., Wang, Y., Mikhail, A., et al. Murine p38-δ mitogen-activated protein kinase, a developmentally regulated protein kinase that is activated by stress and proinflammatory cytokines. J Biol Chem 274(11) 7095-7102 (1999).

Johnson, G.L., and Lapadat, R. Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases. Science 298 1911-1912 (2002).

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10010374-1EA
Cell-Based Assay Fixative 1 ea
Cell-Based Assay Blocking Solution 1 ea
DyLightTM 549-Conjugated Goat Anti-Rabbit Secondary Antibody 1 ea
p38 MAPK (Phospho-Thr180/Tyr182) Primary Antibody 1 ea
Cell-Based Assay Thrombin 1 ea
Size Price Quantity Subtotal
1 ea $330.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-02-12. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample p38 MAPK (Phospho-Thr180/Tyr182) Cell-Based Phosphorylation/Translocation Assay Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

Related Products

Cell-Based Assay Thrombin
ERK/MAPK (Phospho-Thr202/Tyr204) Cell-Based Phosphorylation/Translocation Assay Kit
Goat Anti-Rabbit IgG FITC
p38 MAPK Monoclonal Antibody (Clone 9F12)
p38 MAPK (Phospho-Thr180/Tyr182) Polyclonal Antibody
p38 MAPK (Phospho-Thr180/Tyr182) Primary Antibody
SREBP-2 Cell-Based Translocation Assay Kit
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