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ent-Prostaglandin F2α EIA Kit

Cayman Chemical Item Number 10010382

ent-PGF2α EIA Kit

ent-Prostaglandin F2α EIA Kit

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Description

Prostaglandin F2α (PGF2α) is one of the five primary PGs derived from PGH2 via the cyclooxygenase (COX) pathway. It has many well known biological functions, including important roles in reproduction and smooth muscle contraction and is found at relatively high levels in urine. Yin et al. recently demonstrated that the majority of PGF2α in urine is formed non-enzymatically, as its formation cannot be blocked by inhibitors of COX activity.1 Careful analysis using chiral LC and GC-MS demonstrated that much of the urinary PGF2α is the enantiomer of PGF2α, ent-PGF2α. In healthy volunteers, the ratio of ent-PGF2α:PGF2α in urine is approximately 1.5:1 (0.62 ng/mg creatinine of ent-PGF2α:0.39 ng/mg creatinine of PGF2α). Under conditions of oxidant stress such as that induced by either cigarette smoking or hypercholesterolemia, ent-PGF2α increases disproportionately in relation to PGF2α.1 Cayman’s ent-PGF2α Assay is a competitive EIA that can be used for quantification of ent-PGF2α in urine and other sample matrices. The EIA typically displays an IC50 (50% B/B0) of approximately 100 pg/ml and a detection limit (80% B/B0) of approximately 20 pg/ml.

1 Greco, A., Minghetti, L., and Levi, G. Isoprostanes, novel markers of oxidative injury, help understanding the pathogenesis of neurodegenerative diseases. Neurochem Res 25(9/10) 1357-1364 (2000).

Synonyms
  • ent-PGF2α EIA Kit
Stability 1 year
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere
Specificity
ent-Prostaglandin F2α 100%
ent-Prostaglandin E2 1.6%
Prostaglandin F3α 0.5%
Prostaglandin D2 <0.01%
8-iso Prostaglandin E2 <0.01%
Prostaglandin F1α <0.01%
Prostaglandin F2α <0.01%
11β-Prostaglandin F2α <0.01%
8-iso Prostaglandin F2α <0.01%
8-iso Prostaglandin F2β <0.01%
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Background Reading

Greco, A., Minghetti, L., and Levi, G. Isoprostanes, novel markers of oxidative injury, help understanding the pathogenesis of neurodegenerative diseases. Neurochem Res 25(9/10) 1357-1364 (2000).

Praticò, D., Lawson, J.A., Rokach, J., et al. The isoprostanes in biology and medicine. Trends Endocrinol Metab 12(6) 243-247 (2001).

Smith, R. Parturition. N Engl J Med 356 271-283 (2007).

Heaslip, R.J., and Sickels, B.D. Evidence that prostaglandins can contract the rat aorta via a novel protein kinase C-dependent mechanism. J Pharmacol Exp Ther 250 44-51 (1989).

Leonhardt, A., Busch, C., Schweer, H., et al. Reference intervals and developmental changes in urinary prostanoid excretion in healthy newborns, infants and children. Acta Paediatr 81 191-196 (1992).

Yin, H., Gao, L., Tai, H., et al. Urinary prostaglandin F2α is generated from the isoprostane pathway and not the cyclooxygenase in humans. J Biol Chem 282(1) 329-336 (2007).

Ciabattoni, G., Pugliese, F., Spaldi, M., et al. Radioimmunoassay measurement of prostaglandins E2 and F2α in human urine. J Endocrinol Invest 2 173-182 (1979).

Bennett, A., Hensby, C.N., Sanger, G.J., et al. Metabolites of arachidonic acid formed by human gastrointestinal tissues and their actions on the muscle layers. Br J Pharmacol 74 435-444 (1981).

Arslan, A., and Zingg, H.H. Regulation of COX-2 gene expression in rat uterus in vivo and in vitro. Prostaglandins 52 463-481 (1996).

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10010382-480WELL
ent-Prostaglandin F2α EIA Antiserum 500 dtn
ent-Prostaglandin F2α AChE Tracer 500 dtn
ent-Prostaglandin F2α EIA Standard 1 ea
Precoated (Mouse Anti-Rabbit IgG) EIA 96-Well Strip Plate 5 ea
Polysorbate 20 1 ea
Ellman's Reagent 6 × 250 dtn
EIA Buffer Concentrate (10X) 4 × 10 mL
Wash Buffer Concentrate (400X) 12.5 mL
10010382-96WELL
ent-Prostaglandin F2α EIA Antiserum 100 dtn
ent-Prostaglandin F2α AChE Tracer 100 dtn
ent-Prostaglandin F2α EIA Standard 1 ea
Precoated (Mouse Anti-Rabbit IgG) EIA 96-Well Strip Plate 1 ea
Polysorbate 20 1 ea
Ellman's Reagent 3 × 100 dtn
EIA Buffer Concentrate (10X) 2 × 10 mL
Wash Buffer Concentrate (400X) 5 mL
Size Price Quantity Subtotal
96 wells $246.00 $0.00
480 wells $923.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

  • All 480 well kits are supplied with a single 500 dtn vial of tracer and antiserum that must be used within the stated time following reconstitution.
  • Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay.

Pricing updated 2012-05-25. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample ent-Prostaglandin F2α EIA Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

Related Products

8-Isoprostane EIA Kit
Aconitase Fluorometric Assay Kit
Ascorbate Assay Kit
ent-8-iso Prostaglandin F2α
iPF2α-VI EIA Kit
Prostaglandin F2α EIA Kit
UltraPure Water
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