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10-Acetyl-3,7-dihydroxyphenoxazine

Cayman Chemical Item Number 10010469

ADHP; Amplex Red®; A 6550 (CAS 119171-73-2)

10-Acetyl-3,7-dihydroxyphenoxazine (CAS 119171-73-2)

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Description

10-Acetyl-3,7-dihydroxyphenoxazine (ADHP) is a highly sensitive, stable substrate for horseradish peroxidase (HRP) that enables selective detection of H2O2.1 This colorless, non-fluorescent reagent reacts with H2O2 to produce the fluorescent compound resorufin, which can be analyzed using an excitation wavelength of 520-550 nm and an emission wavelength of 585-595 nm. In a 96-well plate format, ADHP enables detection of H2O2 at a concentration as low as 5 pmol per 100 µl sample.1

1 Zhou, M., Diwu, Z., Panchuk-Voloshina, N., et al. A stable nonfluorescent derivative of resorufin for the fluorometric determination of trace hydrogen peroxide: Applications in detecting the activity of phagocyte NADPH oxidase and other oxidases. Anal Biochem 253 162-168 (1997).

Synonyms
  • ADHP
  • Amplex Red®
  • A 6550
Formal Name 1-​(3,​7-​dihydroxy-​10H-​phenoxazin-​10-​yl)-​ethanone
CAS Number 119171-73-2
Molecular Formula C14H11NO4
Formula Weight 257.2
Formulation A crystalline solid
Purity ≥98%
Stability 2 years
Storage -20°C
Shipping Room temperature in continental US; may vary elsewhere
SMILES
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Oc1ccc2c(c1)​Oc1cc(O)​ccc1N2C(=O)​C

Background Reading

Zhou, M., Diwu, Z., Panchuk-Voloshina, N., et al. A stable nonfluorescent derivative of resorufin for the fluorometric determination of trace hydrogen peroxide: Applications in detecting the activity of phagocyte NADPH oxidase and other oxidases. Anal Biochem 253 162-168 (1997).

Size Price Quantity Subtotal
1 mg $11.00 $0.00
5 mg $50.00 $0.00
10 mg $88.00 $0.00
25 mg $193.00 $0.00
Bulk Contact
Cart Total $0.00

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Pricing updated 2012-05-25. Prices are subject to change without notice.

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Warning This product is not for human or veterinary use.

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