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Palmitoyl thio-PC

Cayman Chemical Item Number 10010521

(CAS 113881-60-0)

Palmitoyl thio-PC (CAS 113881-60-0)

See more	Colorimetric Assay (52) Enzyme Substrates (8) Inflammation (383)

Description

Thioester analogs of glycerophospholipids, in combination with Ellman’s reagent, are convenient colorimetric substrates for the measurement of phospholipase (PL) activity.^1,2 Palmitoyl thio-PC is a chromogenic PLA₂ substrate that contains a palmitoyl thioester at the sn-2 position of the glycerol backbone. Hydrolysis of the thioester by PLA₂ yields a free thiol that reacts readily with DTNB (Ellman’s reagent) giving a bright yellow product with an absorbance maximum at 412 nm. Palmitoyl thio-PC has been used to measure bee venom sPLA₂ activity in a phospholipid:Triton X-100 mixed micelle system.³

1 Hendrickson, H.S., Hendrickson, E.K., and Dybvig, R.H. Chiral synthesis of a dithiolester analog of phosphatidylcholine as a substrate for the assay of phospholipase A₂. J Lipid Res 24 1532-1537 (1983).

2 Reynolds, L.J., Hughes, L.L., and Dennis, E.A. Analysis of human synovial fluid phospholipase A₂ on short chain phosphatidylcholine-mixed micelles: Development of a spectrophotometric assay suitable for a microtiterplate reader. Anal Biochem 204 190-197 (1992).

3 Balet, C., Clingman, K.A., and Hajdu, J. 1-palmitoyl-2-thiopalmitoyl phosphatidylcholine, a highly specific chromogenic substrate of phospholipase A₂. Biochem Biophys Res Commun 150(2) 561-567 (1988).

Formal Name	1-O-hexadecanoyl-2-thio-R-(hexadecanoyl)-sn-glycerol-3-phosphocholine
CAS Number	113881-60-0
Molecular Formula	C₄₀H₈₀NO₇PS
Formula Weight	750.1
Formulation	A crystalline solid
Purity	≥98%
Stability	2 years
Storage	-20°C
Shipping	Room temperature in continental US; may vary elsewhere
SMILES	Copy SMILES to clipboard CCCCCCCCCCCCCCCCC(=O)S[C@H](COC(=O)CCCCCCCCCCCCCCC)COP(=O)(O)O.CCC[N](C)(C)C

Background Reading

Hendrickson, H.S., Hendrickson, E.K., and Dybvig, R.H. Chiral synthesis of a dithiolester analog of phosphatidylcholine as a substrate for the assay of phospholipase A₂. J Lipid Res 24 1532-1537 (1983).

Reynolds, L.J., Hughes, L.L., and Dennis, E.A. Analysis of human synovial fluid phospholipase A₂ on short chain phosphatidylcholine-mixed micelles: Development of a spectrophotometric assay suitable for a microtiterplate reader. Anal Biochem 204 190-197 (1992).

Balet, C., Clingman, K.A., and Hajdu, J. 1-palmitoyl-2-thiopalmitoyl phosphatidylcholine, a highly specific chromogenic substrate of phospholipase A₂. Biochem Biophys Res Commun 150(2) 561-567 (1988).

Size	Price	Quantity	Subtotal
1 mg	$92.00		$0.00
5 mg	$414.00		$0.00
10 mg	$736.00		$0.00
50 mg	$3,220.00		$0.00
Bulk	Contact
		Cart Total	$0.00

Pricing updated 2012-02-12. Prices are subject to change without notice.

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Warning This product is not for human or veterinary use.

Related Products

Arachidonoyl thio-PC

Heptanoyl thio-PC

sPLA₂ (human recombinant Type V)

sPLA₂ (Type III)

Show all 4 Hide all but first 3

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