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ERK/MAPK (Phospho-Thr202/Tyr204) Cell-Based Phosphorylation/Translocation Assay Kit

Cayman Chemical Item Number 10010549

ERK/MAPK (Phospho-Thr202/Tyr204) Cell-Based Phosphorylation/Translocation Assay Kit

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Description

Extracellular signal-regulated kinases (ERKs) 1 and 2 (ERK1/2) are members of the mitogen-activated protein kinase (MAPK) family of cell signalling enzymes controlling cell fates such as embryogenesis, cell differentiation, cell proliferation, and cell death. ERK1/2 are activated via dual phosphorylation on specific tyrosine (Tyr204) and threonine (Thr202) residues by mitogen-activated or extracellular signal-regulated protein kinase kinase (MEK). Cayman’s ERK/MAPK (Phospho-Thr202/Tyr204) Phosphorylation/Translocation Cell-Based Assay Kit provides the tools necessary to study ERK/MAPK phosphorylation and translocation within whole cells. The kit contains a phospho-specific ERK/MAPK (Phospho-Thr202 and Tyr204) primary antibody together with a DyLight™ (product of Thermo Fisher Scientific Inc.) conjugated secondary antibody in a ready-to-use format. Tamoxifen, which has been shown by scientists at Cayman Chemical to cause the translocation of phosphorylated ERK/MAPK (Phospho-Thr202/Tyr204) between the cytoplasm and nuclear compartments, is included as a positive control.
Stability 1 year
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere

Background Reading

Pearson, G., Robinson, F., Gibson, B.T., et al. Mitogen-activated protein (MAP) kinase pathways: Regulation and physiological functions. Endocr Rev 22(2) 153-183 (2001).

Roux, P.P., and Blenis, J. ERK and p38 MAPK-activated protein kinases: A family of protein kinases with diverse biological functions. Microbiol Mol Biol Rev 68(2) 320-344 (2004).

Ebisuya, M., Kondoh, K., and Nishida, E. The duration, magnitude and compartmentalization of ERK MAP kinase activity: mechanisms for providing signaling specificity. J Cell Sci 118 2997-3002 (2005).

Pouysségue, J., and Lenormand, P. Fidelity and spatio-temporal control in MAP kinase (ERKs) signalling. Eur J Biochem 270 3291-3299 (2003).

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10010549-1EA
Cell-Based Assay Fixative 1 ea
Cell-Based Assay Blocking Solution 1 ea
DyLightTM 549-Conjugated Goat Anti-Rabbit Secondary Antibody 1 ea
ERK/MAPK (Phospho-Thr202/Tyr204) Primary Antibody 1 ea
Cell-Based Tamoxifen (100 mM) 1 ea
Size Price Quantity Subtotal
1 ea $330.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-05-25. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample ERK/MAPK (Phospho-Thr202/Tyr204) Cell-Based Phosphorylation/Translocation Assay Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

Related Products

ERK/MAPK (Phospho-Thr202/Tyr204) Polyclonal Antibody
Goat Anti-Rabbit IgG FITC
p38 MAPK (Phospho-Thr180/Tyr182) Cell-Based Phosphorylation/Translocation Assay Kit
p38 MAPK (Phospho-Thr180/Tyr182) Polyclonal Antibody
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Cayman Chemical is a manufacturer, supplier and vendor of biochemical reagents, assay kits, antibodies, and proteins.

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