ERK/MAPK (Phospho-Thr202/Tyr204) Cell-Based Phosphorylation/Translocation Assay Kit || Cayman Chemical

ERK/MAPK (Phospho-Thr²⁰²/Tyr²⁰⁴) Cell-Based Phosphorylation/Translocation Assay Kit

Cayman Chemical Item Number 10010549

ERK/MAPK (Phospho-Thr202/Tyr204) Cell-Based Phosphorylation/Translocation Assay Kit

Description

Extracellular signal-regulated kinases (ERKs) 1 and 2 (ERK1/2) are members of the mitogen-activated protein kinase (MAPK) family of cell signalling enzymes controlling cell fates such as embryogenesis, cell differentiation, cell proliferation, and cell death. ERK1/2 are activated via dual phosphorylation on specific tyrosine (Tyr²⁰⁴) and threonine (Thr²⁰²) residues by mitogen-activated or extracellular signal-regulated protein kinase kinase (MEK). Cayman’s ERK/MAPK (Phospho-Thr²⁰²/Tyr²⁰⁴) Phosphorylation/Translocation Cell-Based Assay Kit provides the tools necessary to study ERK/MAPK phosphorylation and translocation within whole cells. The kit contains a phospho-specific ERK/MAPK (Phospho-Thr²⁰² and Tyr²⁰⁴) primary antibody together with a Dylight (product of Pierce Biotechnology Inc.) conjugated secondary antibody in a ready-to-use format. Tamoxifen, which has been shown by scientists at Cayman Chemical to cause the translocation of phosphorylated ERK/MAPK (Phospho-Thr²⁰²/Tyr²⁰⁴) between the cytoplasm and nuclear compartments, is included as a positive control.

Stability

1 year

Storage

-20°C

Shipping

Wet ice in continental US; may vary elsewhere

Background Reading

Pearson, G., Robinson, F., Gibson, B.T., et al. Mitogen-activated protein (MAP) kinase pathways: Regulation and physiological functions. Endocr Rev 22(2) 153-183 (2001).

Roux, P.P., and Blenis, J. ERK and p38 MAPK-activated protein kinases: A family of protein kinases with diverse biological functions. Microbiol Mol Biol Rev 68(2) 320-344 (2004).

Ebisuya, M., Kondoh, K., and Nishida, E. The duration, magnitude and compartmentalization of ERK MAP kinase activity: mechanisms for providing signaling specificity. J Cell Sci 118 2997-3002 (2005).

Pouysségue, J., and Lenormand, P. Fidelity and spatio-temporal control in MAP kinase (ERKs) signalling. Eur J Biochem 270 3291-3299 (2003).

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10010549-1EA

Cell-Based Assay Fixative

1 ea

Cell-Based Assay Blocking Solution

1 ea

DyLightTM 549-Conjugated Goat Anti-Rabbit Secondary Antibody

1 ea

ERK/MAPK (Phospho-Thr202/Tyr204) Primary Antibody

1 ea

Cell-Based Tamoxifen (100 mM)