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Albumin (bovine) Acetylated

Cayman Chemical Item Number 10010566

(Ac)BSA (CAS 90488-46-8)

See more	EIA (Enzyme Immunoassay) (200) Western Blot (557)

Description

Application(s): ELISA and WB · Acetylated BSA may be used as a positive control for ELISA or WB when employing an acetyl-lysine monoclonal or polyclonal antibody. Cayman’s Acetyl Lysine Monoclonal Antibody (clone 7F8) (Catalog No. 10010567) can detect less than 1 ng of this positive control by immunoblotting. Dilutions from a 1 mg/ml stock solution and 2X Laemmli Buffer may be prepared for WB use.¹ For example, dissolve the 1 mg lyophilized protein in 1 ml of purified water (aliquot and freeze for later, use as needed), then make ten-fold dilutions until the desired concentration range is obtained. Finally dilute the product 1:1 in 2X Laemmli Buffer. Incubate the prepared sample for five minutes in boiling water followed by five minutes on ice prior to loading gels.Histone subunit modifications such as lysine acetylation are regulated by the activity of histone acetyltransferases (HATs) and histone deacetylases (HDACs).^2,3 Epigenetic modifications such as protein acetylation directly influence cellular genetic programs including those contributing to cancer cell viability.^4,2,5

1 Laemmli, U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 680-685 (1970).

2 Loidl, P. Histone acetylation: Facts and questions. Chromosoma 103 441-449 (1994).

3 Cheung, W.L., Briggs, D.B., and Allis, C.D. Acetylation and chromosomal functions. Curr Opin Cell Biol 12 326-333 (2000 Jan 1).

4 Marks, P.A., and Breslow, R. Dimethyl sulfoxide to vorinostat: Development of this histone deacetylase inhibitor as an anticancer drug. Nat Biotech 25(1) 84-90 (2007).

5 Bolden, J.E., Peart, M.J., and Johnstone, R.W. Anticancer activities of histone deacetylase inhibitors. Nat Rev Drug Discov 5 769-784 (2006).

Synonyms	(Ac)BSA
CAS Number	90488-46-8
Formulation	1 mg of acetylated bovine serum albumin (BSA), lyophilized from 50 mM borate, pH 8.5
Stability	2 years
Storage	-20°C
Shipping	Wet ice in continental US; may vary elsewhere

Background Reading

Cheung, W.L., Briggs, D.B., and Allis, C.D. Acetylation and chromosomal functions. Curr Opin Cell Biol 12 326-333 (2000 Jan 1).

Laemmli, U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 680-685 (1970).

Bolden, J.E., Peart, M.J., and Johnstone, R.W. Anticancer activities of histone deacetylase inhibitors. Nat Rev Drug Discov 5 769-784 (2006).

Marks, P.A., and Breslow, R. Dimethyl sulfoxide to vorinostat: Development of this histone deacetylase inhibitor as an anticancer drug. Nat Biotech 25(1) 84-90 (2007).

Loidl, P. Histone acetylation: Facts and questions. Chromosoma 103 441-449 (1994).

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Size	Price	Quantity	Subtotal
1 ea	$26.00		$0.00
Bulk	Contact
		Cart Total	$0.00

Pricing updated 2012-02-12. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

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Cayman Chemical is a manufacturer, supplier and vendor of biochemical reagents, assay kits, antibodies, and proteins.

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