Acetyl Lysine Monoclonal Antibody (Clone 7F8)

Cayman Chemical Item Number 10010567

Description

Antigen: Acetylated KLH · Clone designation: 7F8 · Host: Mouse · Isotype: IgG₁ · Application(s): ELISA, WB, and ICC · This antibody is useful for monitoring levels of acetylation (i.e., histone and p53).¹ Histone subunit modifications such as lysine acetylation are regulated by the activity of histone acetyltransferases (HATs) and histone deacetylases (HDACs).^2,3 Epigenetic modifications directly influence cellular genetic programs including those contributing to cancer cell viability.^4,2,5 This monoclonal antibody was generated using acetylated KLH as an immunogen.

1 Luo, J., Nikolaev, A.Y., Imai, S., et al. Negative control of p53 by Sir2α promotes cell survival under stress. Cell 107 137-148 (2001).

2 Loidl, P. Histone acetylation: Facts and questions. Chromosoma 103 441-449 (1994).

3 Cheung, W.L., Briggs, D.B., and Allis, C.D. Acetylation and chromosomal functions. Curr Opin Cell Biol 12 326-333 (2000 Jan 1).

4 Marks, P.A., and Breslow, R. Dimethyl sulfoxide to vorinostat: Development of this histone deacetylase inhibitor as an anticancer drug. Nat Biotech 25(1) 84-90 (2007).

5 Bolden, J.E., Peart, M.J., and Johnstone, R.W. Anticancer activities of histone deacetylase inhibitors. Nat Rev Drug Discov 5 769-784 (2006).

Formulation

100 µg of purified IgG1 lyophilized from 100 µl of PBS

Stability

1 year

Storage

-20°C

Shipping

Wet ice in continental US; may vary elsewhere

Specificity

(+) Acetylated lysine residues;	+
(-) Non-acetylated lysine residues	-

Background Reading

Bolden, J.E., Peart, M.J., and Johnstone, R.W. Anticancer activities of histone deacetylase inhibitors. Nat Rev Drug Discov 5 769-784 (2006).

Marks, P.A., and Breslow, R. Dimethyl sulfoxide to vorinostat: Development of this histone deacetylase inhibitor as an anticancer drug. Nat Biotech 25(1) 84-90 (2007).

Cheung, W.L., Briggs, D.B., and Allis, C.D. Acetylation and chromosomal functions. Curr Opin Cell Biol 12 326-333 (2000 Jan 1).

Loidl, P. Histone acetylation: Facts and questions. Chromosoma 103 441-449 (1994).

Luo, J., Nikolaev, A.Y., Imai, S., et al. Negative control of p53 by Sir2α promotes cell survival under stress. Cell 107 137-148 (2001).

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1 ea

$295.00

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