Apoptotic Blebs Assay Kit

Cayman Chemical Item Number 10010750

Apoptotic Blebs Assay Kit

Description

One of the hallmark features of cells undergoing apoptosis is the formation of membrane blebs in which parts of the plasma membrane protrude, eventually resulting in cells that look like raspberries. As part of the process, membrane phospholipids such as phosphatidylserine and phosphatidylethanolamine redistribute from the inner to outer leaflet of the membrane bilayer where they are exposed on the cell surface. In addition, some nuclear autoantigens translocate to the cell periphery and become clustered in blebs. Cayman’s Apoptotic Blebs Assay Kit employs a recombinant protein derived from single-chain variable fragments (scFv) of an antibody which preferentially binds to the autoantigen in membrane blebs of apoptotic cells. This recombinant protein is fused to protein A allowing visualiztion using fluorescein-conjugated rabbit IgG. Since membrane blebbing occurs only in cells committed to cell death, this assay is specific for cells in the execution stage of apoptosis. Propidium iodide is included in the kit for detection of dead cells, through either a necrotic or apoptotic pathway. The kit is easy to use and can be easily adapted to high throughput screening for therapeutic compounds regulating apoptosis.

Stability

6 months

Storage

4°C

Shipping

Wet ice in continental US; may vary elsewhere

Background Reading

Bonfoco, E., Krainc, D., Ankarcrona, M., et al. Apoptosis and necrosis: Two distinct events induced, respectively, by mild and intense insults with N-methyl-D-aspartate or nitric oxide/superoxide in cortical cell cultures. Proc Natl Acad Sci USA 92 7162-7166 (1995).

Cocca, B.A., Cline, A.M., and Radic, M.Z. Blebs and apoptotic bodies are B cell autoantigens. J Immunol 169 159-166 (2002).

Schiller, M., Bekerdjian-Ding, I., Heyder, P., et al. Autoantigens are translocated into small apoptotic bodies during early stages of apoptosis. Cell Death Differ 15 183-191 (2008).

Shiratsuchi, A., Mori, T., Takahashi, Y., et al. A presumed human nuclear autoantigen that translocates to plasma membrane blebs during apoptosis. J Biochem 133(2) 211-218 (2003).

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10010750-1EA

Cell-Based Assay Buffer Tablet

4 ea

Single-Chain Variable Fragments (scFv) Fusion Protein

1 ea

Fluorescein-Labeled Rabbit IgG

1 ea

Cell-Based Propidium Iodide Solution

1 ea

Size

Price