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Please feel free to continue placing orders via our website or via fax at 734-971-3640. You may send an email to customer service at custserv@caymanchem.com , or to technical support at techserv@caymanchem.com which we will respond to the next business day. Cayman will resume regular business hours and shipping schedules on Tuesday, May 29, 2012. Thank you for your patience and understanding.

Glycerol Colorimetric Assay Kit

Cayman Chemical Item Number 10010755

Glycerol Colorimetric Assay Kit

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Description

Glycerol is the backbone of triglycerides and an important intermediate in energy metabolism being involved in both oxidative and synthetic processes. The measurement of circulating levels of glycerol and free fatty acids are considered to reflect lipolysis and are therefore useful parameters to evaluate in various conditions. The measurements of glycerol is also useful for correction of glycerol interference when measuring triglycerides in reference materials and in serum from patients with elevated glycerol concentrations. Cayman’s Glycerol Assay provides a simple, reproducible, and sensitive tool for measurement of glycerol in plasma and serum. The assay employs a coupled enzymatic reaction system that yields a brilliant purple product with an absorbance maximum at 540 nm.
Stability 1 year
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere

Background Reading

McCabe, E.R.B. Disorders of glycerol metabolism. 7 1631-1652 (1995).

Jellum, E., and Björnstad, P. Quantitative gas-liquid chromatographic determination of free glycerol in blood serum. J Lipid Res 5 314-317 (1964).

Arner, P. Techniques for the measurement of white adipose tissue metabolism: a practical guide. Int J Obes Relat Metab Disord 19(7) 435-442 (1995).

Bernert, J.T., Bell, C.J., McGuffey, J.E., et al. Determination of “free” glycerol in human serum reference materials by isotope-dilution gas chromatography-mass spectrometry. J Chromatogr 578 1-7 (1992).

Bortz, W.M., Paul, P., Haff, A.C., et al. Glycerol turnover and oxidation in man. J Clin Invest 51(6) 1537-1546 (1972).

Ou, C., and Frawley, V.L. Enzymatic measurement of serum glycerol using a COBAS-BIO centrifugal analyzer. Clin Biochem 18(11) 37-39 (1985).

Li, R., Keymeulen, B., and Gerlo, E. Determination of glycerol in plasma by an automated enzymatic spectrophotometric procedure. Clin Chem Lab Med 39(1) 20-24 (2001).

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10010755-96WELL
Glycerol Standard 1 ea
Glycerol Enzyme Mixture 1 ea
96-Well Solid Plate (Colorimetric Assay)
Sodium Phosphate Assay Buffer 4 mL
Standard Diluent Assay Reagent (5X) 4 mL
Size Price Quantity Subtotal
96 wells $198.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-05-25. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Glycerol Colorimetric Assay Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

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Cayman Chemical Company · 1180 East Ellsworth Road · Ann Arbor, Michigan 48108 · USA

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