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SREBP-1 Transcription Factor Assay Kit

Cayman Chemical Item Number 10010854

Sterol Regulatory Element-Binding Protein-1 Assay Kit

SREBP-1 Transcription Factor Assay Kit

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Synonyms
  • Sterol Regulatory Element-Binding Protein-1 Assay Kit
Stability 1 year
Storage -80°C
Shipping Dry ice in continental US; may vary elsewhere

Description

Three known isoforms of SREBP (sterol regulatory element binding protein) transcription factors have been characterized: SREBP-1a, SREBP-1c, and SREBP-2. SREBP-1c acts primarily to activate genes required for fatty acid synthesis, such as acetyl CoA carboxylase, fatty acid synthase, and long chain fatty acid elongase. In addition, SREBP-1c may also contribute to the regulation of glucose uptake and synthesis through induction of glucokinase. SREBP-1c has important clinical implications in the treatment of many diseases including obesity, diabetes mellitus, insulin resistance, and non-alcoholic fatty liver disease (NAFLD). Cayman’s SREBP-1 Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity. SREBP-1 contained in nuclear extracts and whole cell lysates binds to a dsDNA SREBP response element immobilized to the wells of a 96-well plate. SREBP-1 is detected by addition of a specific primary antibody directed against SREBP-1. A secondary antibody conjugated to HRP is used to provide a sensitive colorimetric readout at 450 nm.

Related Articles

Horton, J.D., Shah, N.A., Warrington, J.A., et al. Combined analysis of oligonucleotide microarray data from transgenic and knockout mice identifies direct SREBP target genes. Proc Natl Acad Sci USA 100(21) 12027-12032 (2003).

Shimano, H., Horton, J.D., Shimomura, I., et al. Isoform 1c of sterol regulatory element binding protein is less active than isoform 1a in livers of transgenic mice and in cultured cells. J Clin Invest 99(5) 846-854 (1997).

Espenshade, P.J., Hughes, A.L. Regulation of sterol synthesis in eukaryotes. Annu Rev Genet 41 401-427 (2007).

Ahmed, M.H., Byrne, C.D. Modulation of sterol regulatory element binding proteins (SREBPs) as potential treatments for non-alcoholic fatty liver disease (NAFLD). Drug Discov Today 12(17/18), 740-747 (2007).

Eberlé, D., Hegarty, B., Bossard, P., et al. SREBP transcription factors: Master regulators of lipid homeostasis. Biochimie 86 839-848 (2004).

Shimano, H. SREBP-1c and TFE3, energy transcription factors that regulate hepatic insulin signaling. J Mol Med 85 437-444 (2007).

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10010854-96well
Transcription Factor Binding Assay Buffer (4X) 1 ea
Transcription Factor Antibody Binding Buffer (10X) 1 ea
Transcription Factor Goat Anti-Rabbit HRP Conjugate 1 ea
Transcription Factor Developing Solution 1 ea
Transcription Factor Stop Solution 1 ea
Transcription Factor Reagent A 1 ea
Transcription Factor SREBP 96-Well Strip Plate 1 ea
Transcription Factor SREBP Specific Competitor dsDNA 1 ea
Transcription Factor SREBP-1 Positive Control 1 ea
Transcription Factor SREBP-1 Primary Antibody 1 ea
Tween 20 1 ea
Wash Buffer Concentrate (400X) 5 mL

Pricing updated 2010-09-06. Prices are subject to change without notice.

Pricing is for North America only. Other customers should contact a distributor in their region.

Cayman strives to be a reliable biochemical reagent vendor by providing the best possible products and services. To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample SREBP-1 Transcription Factor Assay Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

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