Luminex® 11-dehydro Thromboxane B2 Kit

Luminex^® 11-dehydro Thromboxane B₂ Kit

Cayman Chemical Item Number 10010971

Luminex^® 11-dehydro TXB₂ Kit

Description

Limit of detection: 80% B/B₀: 130 pg/ml · Sensitivity: 50% B/B₀: 650 pg/ml · Thromboxane A₂ (TXA₂) is produced from arachidonic acid by many cells and causes irreversible platelet aggregation and contraction of vascular and bronchial smooth muscle. TXA₂ is rapidly hydrolyzed non-enzymatically to form TXB₂. 11-dehydro TXB₂ is a metabolite of TXB₂ which can be measured in urine to reflect systemic production of TXB₂ in vivo. Cayman’s Luminex^® 11-dehydro TXB₂ Kit is the first of its kind for the measurement of 11-dehydro TXB₂ using Luminex^® xMAP^® technology. For application of this technology to the measurement of 11-dehydro TXB₂ in a competitive format, microspheres have been coated with an 11-dehydro TXB₂ monoclonal antibody. The assay is based on the competition between 11-dehydro TXB₂ and a 11-dehydro TXB₂-phycoerytherin conjugate (11-dehydro TXB₂ tracer) for the monoclonal antibody binding sites on the microsphere beads. After sufficient equilibration time the assays are read using a Luminex^® 100TM or 200TM analyzer.

Synonyms

Luminex® 11-dehydro TXB2 Kit

Stability

1 year

Storage

-20°C

Shipping

Wet ice in continental US; may vary elsewhere

Background Reading

Hamberg, M., Svensson, J., and Samuelsson, B. Thromboxanes: A new group of biologically active compounds derived from prostaglandin endoperoxides. Proc Natl Acad Sci USA 72 2994-2998 (1975).

Lawson, J.A., Patrono, C., Ciabattoni, G., et al. Long-lived enzymatic metabolites of thromboxane B₂ in the human circulation. Anal Biochem 155 198-205 (1986).

Ciabattoni, G., Pugliese, F., Davi, G., et al. Fractional conversion of thromboxane B₂ to urinary 11-dehydrothromboxane B₂ in man. Biochim Biophys Acta 992 66-70 (1989).

Roberts, L.J., Sweetman, B.J., and Oates, J.A. Metabolism of thromboxane B₂ in man. J Biol Chem 256(16) 8384-8393 (1981).

Patrono, C., Ciabattoni, G., Pugliese, F., et al. Estimated rate of thromboxane secretion into the circulation of normal humans. J Clin Invest 77 590-594 (1986).

Ellis, E.F., Oelz, O., Roberts, L.J., et al. Coronary arterial smooth muscle contraction by a substance released from platelets: Evidence that it is thromboxane A₂. Science 193 1135-1137 (1976).

Salzman, P.M., Salmon, J.A., and Moncada, S. Prostacyclin and thromboxane A₂ synthesis by rabbit pulmonary artery. J Pharmacol Exp Ther 215 240-247 (1980).

Samuelsson, B., Granström, E., Green, K., et al. Prostaglandins. Annu Rev Biochem 44 669-695 (1975).

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10010971-1EA

Luminex® Assay Buffer Concentrate (10X)

10 mL

11-dehydro Thromboxane B2 xMAP® Beads

1 ea

11-dehydro Thromboxane B2 Phycoerythrin Tracer

1 ea

11-dehydro Thromboxane B2 Luminex® Standard

1 ea

High-Binding 96-Well Strip Plate