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Holiday Notification: Cayman Chemical will be closed Monday, May 28, 2012, in observance of the Memorial Day holiday. More…
Please feel free to continue placing orders via our website or via fax at 734-971-3640. You may send an email to customer service at custserv@caymanchem.com , or to technical support at techserv@caymanchem.com which we will respond to the next business day. Cayman will resume regular business hours and shipping schedules on Tuesday, May 29, 2012. Thank you for your patience and understanding.

LDL Uptake Cell-Based Assay Kit

Cayman Chemical Item Number 10011125

Low-Density Lipoprotein Uptake

LDL Uptake Cell-Based Assay Kit

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Description

Low-density lipoproteins (LDL) is the major carrier of cholesterol in the blood, accounting for more than 60% of total plasma cholesterol. LDL is taken up by hepatic and extrahepatic tissues through LDL receptor-mediated endocytosis where it is internalized and degraded to free cholesterol and amino acids. Uptake of native and oxidized LDL by monocyte-derived macrophages in the vessel wall results in accumulation of cholesterol and formation of atherosclerotic plaques. The mechanism underlying the uptake of LDL by both hepatic tissue and cells of the cardiovascular system is not clearly understood. Thus, LDL uptake and its regulation are important therapeutic targets for atherosclerosis and related diseases. Cayman’s LDL Uptake Cell-Based Assay Kit provides a convenient tool for studying LDL uptake and regulation at cellular level. The kit employs human LDL conjugated to DyLight 549 as a fluorescent probe for detection of LDL uptake into cultured cells. A LDL receptor-specific polyclonal antibody and a DyLight 488-conjugated secondary antibody are included for identifying the distribution of LDL receptors.
Synonyms
  • Low-Density Lipoprotein Uptake
Stability 6 months
Storage 4°C
Shipping Wet ice in continental US; may vary elsewhere

Background Reading

Soccio, R.E., and Breslow, J.L. Intracellular cholesterol transport. Arterioscler Thromb Vasc Biol 24 1150-1160 (2004).

Goldstein, J.L., Brown, M.S., Anderson, R.G.W., et al. Receptor-mediated endocytosis: Concepts emerging from the LDL receptor system. Annu Rev Cell Biol 1 1-39 (1985).

Rudling, M.J., Reihnér, E., Einarsson, K., et al. Low density lipoprotein receptor-binding activity in human tissues: Quantitative importance of hepatic receptors and evidence for regulation of their expression in vivo. Proc Natl Acad Sci USA 87 3469-3473 (1990).

Lester, C., Nikogosian, K., Shrestha, R., et al. Atherosclerotic assault. Eukaryon 2 40-46 (2006).

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10011125-1EA
Cell-Based Assay Fixative 1 ea
Cell-Based Assay Blocking Solution 1 ea
LDL-DyLightTM 549 1 ea
DyLightTM 488-Conjugated Goat Anti-Rabbit IgG Secondary Antibody 1 ea
Rabbit Anti-LDL Receptor Primary Antibody 1 ea
Size Price Quantity Subtotal
1 ea $356.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-05-25. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample LDL Uptake Cell-Based Assay Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

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Cayman Chemical is a manufacturer, supplier and vendor of biochemical reagents, assay kits, antibodies, and proteins.

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