LDL Uptake Cell-Based Assay Kit

Cayman Chemical Item Number 10011125

Low-Density Lipoprotein Uptake

Description

Low-density lipoproteins (LDL) is the major carrier of cholesterol in the blood, accounting for more than 60% of total plasma cholesterol. LDL is taken up by hepatic and extrahepatic tissues through LDL receptor-mediated endocytosis where it is internalized and degraded to free cholesterol and amino acids. Uptake of native and oxidized LDL by monocyte-derived macrophages in the vessel wall results in accumulation of cholesterol and formation of atherosclerotic plaques. The mechanism underlying the uptake of LDL by both hepatic tissue and cells of the cardiovascular system is not clearly understood. Thus, LDL uptake and its regulation are important therapeutic targets for atherosclerosis and related diseases. Cayman’s LDL Uptake Cell-Based Assay Kit provides a convenient tool for studying LDL uptake and regulation at cellular level. The kit employs human LDL conjugated to DyLight^™ 549 as a fluorescent probe for detection of LDL uptake into cultured cells. A LDL receptor-specific polyclonal antibody and a DyLight^™ 488-conjugated secondary antibody are included for identifying the distribution of LDL receptors.

Synonyms

Low-Density Lipoprotein Uptake

Stability

6 months

Storage

4°C

Shipping

Wet ice in continental US; may vary elsewhere

Background Reading

Soccio, R.E., and Breslow, J.L. Intracellular cholesterol transport. Arterioscler Thromb Vasc Biol 24 1150-1160 (2004).

Goldstein, J.L., Brown, M.S., Anderson, R.G.W., et al. Receptor-mediated endocytosis: Concepts emerging from the LDL receptor system. Annu Rev Cell Biol 1 1-39 (1985).

Rudling, M.J., Reihnér, E., Einarsson, K., et al. Low density lipoprotein receptor-binding activity in human tissues: Quantitative importance of hepatic receptors and evidence for regulation of their expression in vivo. Proc Natl Acad Sci USA 87 3469-3473 (1990).

Lester, C., Nikogosian, K., Shrestha, R., et al. Atherosclerotic assault. Eukaryon 2 40-46 (2006).

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10011125-1EA

Cell-Based Assay Fixative

1 ea

Cell-Based Assay Blocking Solution

1 ea

LDL-DyLightTM 549

1 ea

DyLightTM 488-Conjugated Goat Anti-Rabbit IgG Secondary Antibody

1 ea

Rabbit Anti-LDL Receptor Primary Antibody