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Holiday Notification: Cayman Chemical will be closed Monday, May 28, 2012, in observance of the Memorial Day holiday. More…
Please feel free to continue placing orders via our website or via fax at 734-971-3640. You may send an email to customer service at custserv@caymanchem.com , or to technical support at techserv@caymanchem.com which we will respond to the next business day. Cayman will resume regular business hours and shipping schedules on Tuesday, May 29, 2012. Thank you for your patience and understanding.

NF-κB (human p50/p65) Combo Transcription Factor Assay Kit

Cayman Chemical Item Number 10011223

NF-κB (human p50/p65) Combo Transcription Factor Assay Kit

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Description

The NF-κB/Rel family of transcription factors is comprised of several structurally-related proteins that form homodimers and heterodimers and include p50/p105, p52/p100, RelA (p65), and c-Rel/NF-κB. Acting as dimers, these transcription factors bind to κB sites on DNA, thereby regulating expression of target genes. The most common Rel/NF-κB dimer in mammals contains p50-RelA (p50/p65) heterodimers and is an attractive target for potential therapeutics in human inflammation and certain other diseases. Cayman’s NF-κB (human p50/p65) Combo Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts. A specific double stranded DNA (dsDNA) sequence containing the NF-κB response element is immobilized onto the wells of a 96-well plate. NF-κB contained in a nuclear extract, binds specifically to the NF-κB response element. NF-κB p50 and p65 are detected by addition of specific primary antibodies directed against either NF-κB p50 or p65. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm.
Stability 6 months
Storage -80°C
Shipping Dry ice in continental US; may vary elsewhere

Background Reading

Gilmore, T.D. The Rel/NF-κB signal transduction pathway: Introduction. Oncogene 18 6842-6844 (1999).

Arkan, M.C., Hevener, A.L., Greten, F.R., et al. IKK-β links inflammation to obesity-induced insulin resistance. Nat Med 11(2) 191-198 (2005).

Pahl, H.L. Activators and target genes of Rel/NF-κB transcription factors. Oncogene 18 6853-6866 (1999).

Karin, M. The beginning of the end: IκB kinase (IKK) and NF-κB activation. J Biol Chem 274(39) 27339-27342 (1999).

Maeda, S., Hsu, L., Liu, H., et al. Nod2 mutation in Crohn’s disease potentiates NF-κB activity and IL-1β processing. Science 307 734-738 (2005).

Gilroy, D.W., Lawrence, T., Perretti, M., et al. Inflammatory resolution: New opportunities for drug discovery. Nat Rev Drug Discov 3 401-416 (2004).

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10011223-96WELL
Transcription Factor Binding Assay Buffer (4X) 1 ea
Transcription Factor Antibody Binding Buffer (10X) 1 ea
Transcription Factor Goat Anti-Rabbit HRP Conjugate 1 ea
Transcription Factor Developing Solution 1 ea
Transcription Factor Stop Solution 1 ea
Transcription Factor Reagent A 1 ea
Transcription Factor NF-κB 96-Well Strip Plate 1 ea
Transcription Factor NF-κB Competitor dsDNA 1 ea
Transcription Factor NF-κB (human p65) Combo Positive Control 1 ea
Transcription Factor NF-κB (human p50) Combo Positive Control 1 ea
Transcription Factor NF-κB (human p50) Combo Primary Antibody 1 ea
Transcription Factor NF-κB (p65) Combo Primary Antibody 1 ea
Polysorbate 20 1 ea
Wash Buffer Concentrate (400X) 5 mL
Size Price Quantity Subtotal
96 wells $410.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-05-25. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample NF-κB (human p50/p65) Combo Transcription Factor Assay Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

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