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Join us! · InformexUSA 2012 · New Orleans, Louisiana · February 14-17, 2012 · Booth 2514

Protocatechuate Dioxygenase

Cayman Chemical Item Number 10011353

PCD; Protocatechuate 3,4-Dioxygenase; Aromatic Dioxygenase

Protocatechuate Dioxygenase

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Description

Source: recombinant protein expressed in E. coli · Mr: 23 kDa (α-subunit) 26.5 kDa (β-subunit) · Protocatechuate dioxygenase (PCD) catalyzes the proximal extradiol ring cleavage of protocatechuate (PCA) with attendant incorporation of both atoms of oxygen from O2.1 The PCD holoenzyme consists of α (23 kDa) and β (26.5 kDa) subunits, which form a tetramer with four ferric ions for a total weight of ~200 kDa.2 Iron is essential for activity because the substrate, PCA, initially binds directly to the iron through both hydroxyl groups to form a chelate complex.1 PCD is an important component of bacterial metabolism of aromatic compounds found in the soil. However, many publications are now reporting the usefulness of the PCD/PCA oxygen scrubbing (oxygen scavenging) system for maintaining anaerobic experimental conditions.3,4 Cayman’s protocatechuate dioxygenase is the 3,4-PCD expressed and purified from a bacterial overexpression system. 3,4-PCD catalyzes the oxidative ring cleavage of 3,4-dihydroxybenzoate to produce β-carboxy-cis,cis-muconate. Activity of the PCD can be measured by monitoring the rate of O2 uptake with an oxygen electrode, or by measuring the amount of product (β-carboxy-cis,cis-muconate) formed at 290 nm.

1 Wogel, S.A., Dege, J.E., Perkins-Olson, P.E., et al. Purification and characterization of protocatechuate 2,3-dioxygenase from Bacillus macerans: A new extradiol catecholic dioxygenase. J Bacteriol 175(14) 4414-4426 (1993 Jul).

2 Bull, C., and Ballou, D.P. Purification and properties of protocatechuate 3,4-dioxygenase from Pseudomonas putida. J Biol Chem 256(24) 12673-12680 (1981 Dec 25).

3 Patil, P.V., and Ballou, D.P. The use of protocatechuate dioxygenase for maintaining anaerobic conditions in biochemical experiments. Anal Biochem 286 187-192 (2000).

4 Aitken, C.E., Marshall, R.A., and Puglisi, J.D. An oxygen scavenging system for improvement of dye stability in single-molecule fluorescence experiments. Biophys J 94 1826-1835 (2008).

Synonyms
  • PCD
  • Protocatechuate 3,4-Dioxygenase
  • Aromatic Dioxygenase
Formulation 20 mM potassium phosphate, pH 7.0, containing 20% glycerol
Purity ≥90%
Stability 6 months
Storage -80°C
Shipping Dry ice in continental US; may vary elsewhere

Background Reading

Aitken, C.E., Marshall, R.A., and Puglisi, J.D. An oxygen scavenging system for improvement of dye stability in single-molecule fluorescence experiments. Biophys J 94 1826-1835 (2008).

Patil, P.V., and Ballou, D.P. The use of protocatechuate dioxygenase for maintaining anaerobic conditions in biochemical experiments. Anal Biochem 286 187-192 (2000).

Bull, C., and Ballou, D.P. Purification and properties of protocatechuate 3,4-dioxygenase from Pseudomonas putida. J Biol Chem 256(24) 12673-12680 (1981 Dec 25).

Wogel, S.A., Dege, J.E., Perkins-Olson, P.E., et al. Purification and characterization of protocatechuate 2,3-dioxygenase from Bacillus macerans: A new extradiol catecholic dioxygenase. J Bacteriol 175(14) 4414-4426 (1993 Jul).

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Size Price Quantity Subtotal
250 µg $51.00 $0.00
500 µg $97.00 $0.00
1 mg $184.00 $0.00
Bulk Contact
Cart Total $0.00

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Pricing updated 2012-02-12. Prices are subject to change without notice.

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