Antigen: human recombinant Hsp40 · Host: rabbit · Application(s): WB · DnaJ/Hsp40 proteins have been preserved throughout evolution and are important for protein translation, folding, unfolding, translocation, and degradation, primarily by stimulating the ATPase activity of chaperone proteins, Hsp70s. Because the ATP hydrolysis is essential for the activity of Hsp70s, DnaJ/Hsp40 proteins actually determine the activity of Hsp70s by stabilizing their interaction with substrate proteins. DnaJ/Hsp40 proteins all contain the J domain through which they bind to Hsp70s. Hsp40, also known as HDJ1¹, is a basic mammalian 40 kDa heat shock protein which is not only homologous to the bacterial heat shock protein (DnaJ), but also yeast DnaJ-related proteins such as SCJ1, Sec63/Npl1, YDJ1, and SIS1.^2,3,4,5 Hsp40 is inducible by stress including heat after which is moves from the cytoplasm to the nucleus and nucleoli; an intracellular pattern similar to Hsc70/Hsp70, the mammalian homologues of the bacterial heat shock protein, DnaK.¹

1 Ohtsuka, K. Cloning of a cDNA for heat-shock protein hsp40, a human homolog of bacterial dnaJ. Biochem Biophys Res Commun 197 235-240 (1993).

2 Hattori, H., Liu, L., Tohnai, I., et al. Intracellular localization and partial amino acid sequence of a stress-inducible 40-kDa protein in HeLa cells. Cell Struct Funct 17 77-86 (1992).

3 Ohtsuka, K., Masuda, A., Nakai, A., et al. A novel 40-kDa protein induced by heat shock and other stresses in mammalian and avian cells. Biochem Biophys Res Commun 166 642-647 (1990).

4 Bardwell, J.C.A., Tilly, K., Craig, E., et al. The nucleotide sequence of the Escherichia coli K12 dnaJ gene: A gene that encodes a heat shock protein. J Biol Chem 261(4) 1782-1785 (1986).

5 Ohki, M., Tamura, F., Nishimura, S., et al. Nucleotide sequence of the escherichia coli dnaJ gene and purification of the gene product. J Biol Chem 261(4) 1778-1781 (1986).

Melville, M.W., Hansen, W.J., Freeman, B.C., et al. The molecular chaperone hsp40 regulates the activity of P58IPK, the cellular inhibitor of PKR. Proc Natl Acad Sci USA 94 97-102 (1997).

Ohtsuka, K. Cloning of a cDNA for heat-shock protein hsp40, a human homolog of bacterial dnaJ. Biochem Biophys Res Commun 197 235-240 (1993).

Ohtsuka, K., Masuda, A., Nakai, A., et al. A novel 40-kDa protein induced by heat shock and other stresses in mammalian and avian cells. Biochem Biophys Res Commun 166 642-647 (1990).

Hattori, H., Liu, L., Tohnai, I., et al. Intracellular localization and partial amino acid sequence of a stress-inducible 40-kDa protein in HeLa cells. Cell Struct Funct 17 77-86 (1992).

Bardwell, J.C.A., Tilly, K., Craig, E., et al. The nucleotide sequence of the Escherichia coli K12 dnaJ gene: A gene that encodes a heat shock protein. J Biol Chem 261(4) 1782-1785 (1986).

Ohki, M., Tamura, F., Nishimura, S., et al. Nucleotide sequence of the escherichia coli dnaJ gene and purification of the gene product. J Biol Chem 261(4) 1778-1781 (1986).

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