Antigen: internal central human Bim amino acids · Host: rabbit · Application(s): WB and IHC · Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bim/BOD is a group of three splice variants, BimEL, BimL and BimS, with apparent molecular masses of ~23, 16, and 13 kDa, respectively. Bcl-2 homology 3 (BH₃) domain is a potent death domain. BH₃ domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH₃ domain containing protein was recently identified and designated Bim or BOD in human, mouse, and rat.^1,2 Bim/BOD interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. Bim/BOD induces apoptosis. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines.^1,2

1 O'Connor, L., Strasser, A., O'Reilly, L.A., et al. Bim: A novel member of the Bcl-2 family that promotes apoptosis. EMBO J 17 384-395 (1998).

2 Hsu, S.Y., Lin, P., and Hsueh, A.J.W. BOD (Bcl-2-related ovarian death gene) is an ovarian BH₃ domain-containing proapoptotic Bcl-2 protein capable of dimerization with diverse antiapoptotic Bcl-2 members. Mol Endocrinol 12 1432-1440 (1998).

O'Connor, L., Strasser, A., O'Reilly, L.A., et al. Bim: A novel member of the Bcl-2 family that promotes apoptosis. EMBO J 17 384-395 (1998).

Hsu, S.Y., Lin, P., and Hsueh, A.J.W. BOD (Bcl-2-related ovarian death gene) is an ovarian BH₃ domain-containing proapoptotic Bcl-2 protein capable of dimerization with diverse antiapoptotic Bcl-2 members. Mol Endocrinol 12 1432-1440 (1998).