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Antigen:
human Mn SOD
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Host:
rabbit
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Application(s):
WB, IP, and IHC
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Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body.1 It catalyzes the dismutation of the superoxide radical O2- to O2 and H2O2.2,3 There are two main types of SOD in mammalian cells. One form, SOD1, contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cystines forming an intra-subunit disulphide bridge.1 The second form, SOD2, is a manganese-containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form, SOD3 or EC-SOD, is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDa and it exists only in the extra-cellular space.4 SOD3 can also be distinguished by its heparin-binding capacity.5
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1
Furukawa, Y., O'Halloran, T.V. Posttranslational modifications in Cu,Zn-superoxide dismutase and mutations associated with amyotrophic lateral sclerosis. Antioxid Redox Signal 8(5-6) 847-867 (2006).
2
Bannister, J.V., Bannister, W.H., Rotilio, G. Aspects of the structure, function, and applications of superoxide dismutase. Crit Rev Biochem Mol Biol 22(2) 111-180 (1987).
3
Hassan, H.M. Biosynthesis and regulation of superoxide dismutases. Free Radic Biol Med 5(5-6) 377-385 (1988).
4
Wispé, J.R., Clark, J.C., Burhans, M.S., et al. Synthesis and processing of the precursor for human mangano-superoxide dismutase. Biochim Biophys Acta 994(1) 30-36 (1989).
5
Adachi, T., Ohta, H., Yamada, H., et al. Quantitative analysis of extracellular-superoxide dismutase in serum and urine by ELISA with monoclonal antibody. Clin Chim Acta 212(3) 89-102 (1992).
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