See personalized New Product recommendations! Get personalized New Product recommendations! Register or Login for personalized New Product recommendations!

Holiday Notification: Cayman Chemical will be closed Monday, May 28, 2012, in observance of the Memorial Day holiday. More…
Please feel free to continue placing orders via our website or via fax at 734-971-3640. You may send an email to customer service at custserv@caymanchem.com , or to technical support at techserv@caymanchem.com which we will respond to the next business day. Cayman will resume regular business hours and shipping schedules on Tuesday, May 29, 2012. Thank you for your patience and understanding.

CaMKII (Phospho-Thr286/Thr287) Monoclonal Antibody (Clone 22B1)

Cayman Chemical Item Number 10011438

Calcium/Calmodulin-dependent Protein Kinase II

See more

Description

Antigen: synthetic peptide · Clone designation: clone 22B1 · Host: mouse · Isotype: IgG1 · Application(s): ELISA, IF, IP, and WB · CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling.1,2 CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consists of two subunits of 52 (termed α genes) and 60 kDa (β genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site.3,4,5,6,7 The binding of Ca2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase.8 This kinase activation results in autophosphorylation at threonine 286 (Thr286).8 The threonine phosphorylation state of CaMKII can be regulated through protein phosphatase 1 (PP1)/protein kinase A (PKA). Whereas PP1 dephosphorylates phospho-CaMKII at Thr286, PKA prevents this dephosphorylation.9 Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities.10,11,12

1 Hughes, K., Edin, S., Antonsson Å, et al. Calmodulin-dependent kinase II mediates T cell receptor/CD3- and phorbol ester-induced activation of IkB kinase. J Biol Chem 276(38) 36008-36013 (2001).

2 Barria, A., Muller, D., Derkach, V., et al. Regulatory phosphorylation of AMPA-type glutamate receptors by CaM-KII during long-term potentiation. Science 276 2042-2045 (1997).

3 Bennett, M.K., and Kennedy, M.B. Deduced primary structure of the b subunit of brain type II Ca2+/calmodulin-dependent protein kinase determined by molecular cloning. Proc Natl Acad Sci USA 84 1794-1798 (1987).

4 Brocke, L., Srinivassan, M., and Schulman, H. Development and regional expression of multifunctional Ca2+/calmodulin-dependent protein kinase isoforms in rat brain. J Neurosci 15(10) 6797-6808 (1995).

5 Nghiem, P., Saati, S.M., Martens, C.L., et al. Cloning and analysis of two new isoforms of multifunctional Ca2+/calmodulin-dependent protein kinase. Expression in multiple human tissues. J Biol Chem 268(8) 5471-5479 (1983).

6 Edman, C.F., and Schulman, H. Identification and characterization of delta B-CaM kinase and delta C-Cam kinase from rat heart, two new multifunctional Ca2+/calmodulin-dependent protein kinase isoforms. Biochim Biophys Acta 1221(1) 89-101 (1994).

7 Tombes, R.M., and Krystal, G.W. Identification of novel human tumor cell-specific CaMK-II variants. Biochim Biophys Acta 1355(3) 281-292 (1997).

8 Means, A.R. Regulatory cascades involving calmodulin-dependent protein kinases. Mol Endocrinol 14(1) 4-13 (2000).

9 Makhinson, M., Chotiner, J.K., Watson, J.B., et al. Adenylyl cyclase activation modulates activity-dependent changes in synaptic strength and Ca2+/calmodulin-dependent kinase II autophosphorylation. J Neurochem 19(7) 2500-2510 (1999).

10 Strack, S., and Colbran, R.J. Autophosphorylation-dependent targeting of calcium/calmodulin-dependent protein kinase II by the NR2B subunit of the N-methyl-D-aspartate receptor. J Biol Chem 273(33) 20689-20692 (1998).

11 Leonard, A.S., Lim, I.A., Hemsworth, D.E., et al. Calcium/calmodulin-dependent protein kinase II is associated with the N-methyl-D-aspartate receptor. Proc Natl Acad Sci USA 96 3239-3244 (1999).

12 Shen, K., and Meyer, T. Dynamic control of CaMKII translocation and localization in hippocampal neurons by NMDA receptor stimulation. Science 284 162-167 (1999).

Synonyms
  • Calcium/Calmodulin-dependent Protein Kinase II
Formulation Protein G-purified mouse IgG at a concentration of 1 mg/ml in PBS, pH. 7.4, containing 50% glycerol and 0.09% sodium azide
Stability 1 year
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere
Specificity
Rat CaMKII +

Background Reading

Hughes, K., Edin, S., Antonsson Å, et al. Calmodulin-dependent kinase II mediates T cell receptor/CD3- and phorbol ester-induced activation of IkB kinase. J Biol Chem 276(38) 36008-36013 (2001).

Brocke, L., Srinivassan, M., and Schulman, H. Development and regional expression of multifunctional Ca2+/calmodulin-dependent protein kinase isoforms in rat brain. J Neurosci 15(10) 6797-6808 (1995).

Shen, K., and Meyer, T. Dynamic control of CaMKII translocation and localization in hippocampal neurons by NMDA receptor stimulation. Science 284 162-167 (1999).

Leonard, A.S., Lim, I.A., Hemsworth, D.E., et al. Calcium/calmodulin-dependent protein kinase II is associated with the N-methyl-D-aspartate receptor. Proc Natl Acad Sci USA 96 3239-3244 (1999).

Strack, S., and Colbran, R.J. Autophosphorylation-dependent targeting of calcium/calmodulin-dependent protein kinase II by the NR2B subunit of the N-methyl-D-aspartate receptor. J Biol Chem 273(33) 20689-20692 (1998).

Makhinson, M., Chotiner, J.K., Watson, J.B., et al. Adenylyl cyclase activation modulates activity-dependent changes in synaptic strength and Ca2+/calmodulin-dependent kinase II autophosphorylation. J Neurochem 19(7) 2500-2510 (1999).

Means, A.R. Regulatory cascades involving calmodulin-dependent protein kinases. Mol Endocrinol 14(1) 4-13 (2000).

Shifman, J.M., Choi, M.H., Mihalas, S., et al. Ca2+/calmodulin-dependent protein kinase II (CaMKII) is activated by calmodulin with two bound calciums. Proc Natl Acad Sci USA 103(38) 13968-13973 (2006).

Tombes, R.M., and Krystal, G.W. Identification of novel human tumor cell-specific CaMK-II variants. Biochim Biophys Acta 1355(3) 281-292 (1997).

Edman, C.F., and Schulman, H. Identification and characterization of delta B-CaM kinase and delta C-Cam kinase from rat heart, two new multifunctional Ca2+/calmodulin-dependent protein kinase isoforms. Biochim Biophys Acta 1221(1) 89-101 (1994).

Nghiem, P., Saati, S.M., Martens, C.L., et al. Cloning and analysis of two new isoforms of multifunctional Ca2+/calmodulin-dependent protein kinase. Expression in multiple human tissues. J Biol Chem 268(8) 5471-5479 (1983).

Bennett, M.K., and Kennedy, M.B. Deduced primary structure of the b subunit of brain type II Ca2+/calmodulin-dependent protein kinase determined by molecular cloning. Proc Natl Acad Sci USA 84 1794-1798 (1987).

Barria, A., Muller, D., Derkach, V., et al. Regulatory phosphorylation of AMPA-type glutamate receptors by CaM-KII during long-term potentiation. Science 276 2042-2045 (1997).

Show all 13 Hide all but first 3
Size Price Quantity Subtotal
25 µg $115.00 $0.00
100 µg $414.00 $0.00
Bulk Contact
Cart Total $0.00

This product is available in custom sizes and/or larger quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-05-25. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

Related Products

CaMKII Monoclonal Antibody (Clone 6G9)

Downloads

Batch-specific Information

Login to access batch-specific information

Cayman Chemical is a manufacturer, supplier and vendor of biochemical reagents, assay kits, antibodies, and proteins.

Other Resources
Management Team Company Profile Company History ChemAssistant Tools FAQs Cayman Europe Cayman Pharma CaBRI Cayman Gear Press Releases Illustrations and Charts Key Research Area Posters Article Library Analysis Tools Conference Schedule Order Terms Browser Recommendation Privacy Statement Site Map

Cayman Chemical Company · 1180 East Ellsworth Road · Ann Arbor, Michigan 48108 · USA

Toll Free: (800) 364-9897 (USA and Canada Only) · Fax: (734) 971-3640

Copyright 2012 Cayman Chemical Company

Lost password?