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Please feel free to continue placing orders via our website or via fax at 734-971-3640. You may send an email to customer service at custserv@caymanchem.com , or to technical support at techserv@caymanchem.com which we will respond to the next business day. Cayman will resume regular business hours and shipping schedules on Tuesday, May 29, 2012. Thank you for your patience and understanding.

His-Express Detection EIA Kit

Cayman Chemical Item Number 10012445

His-Express Detection EIA Kit

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His-Express Detection EIA Kit Analysis Tools

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Description

Recombinant proteins are routinely labeled with affinity tags, such as hexahistidine (6X-His), GST and FLAG, to facilitate both their detection and purification. 6X-His tags are often the tag of choice due to their small size, less potential to interfere in protein folding, weak immunogenicity, and high affinity for Ni2+. Cell lysates and samples at different stages of purification are generally analyzed by SDS-PAGE, a process which can add several days to the purification and analysis protocol. A semi-quantitative screening assay provides the ability to rapidly assess the levels His-tagged proteins at each stage of the expression and purification process. This permits the user to quickly monitor expression efforts, as well as follow protein loss or enrichment at each purification step. Cayman’s His-Express Detection EIA Kit is competitive assay designed for the rapid, semi-quantitative screening of cell lysates and affinity column fractions for His-tagged proteins. It is intended to serve as a substitute for SDS-PAGE, thereby expediting the screening of affinity column fractions.
Stability 6 months
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere

Background Reading

Bornhorst, J.A., and Falke, J.J. Purification of proteins using polyhistidine affinity tags. Methods Enzymol 326 245-254 (2000).

Debeljak, N., Feldman, L., Davis, K.L., et al. Variability in the immunodetection of His-tagged recombinant proteins. Anal Biochem 359(2) 216-223 (2006).

Andersen, N.H., Subramanian, N., Imamoto, S., et al. Omega chain methylated analogs of PGF2α and PGE2. Prostaglandins 22 809-830 (1981).

10012445-96well
Precoated (Goat Anti-Mouse IgG) EIA 96-Well Strip Plate 1 ea
DEA Buffer Concentrate (10X) 2.5 mL
TBS Assay Buffer Concentrate (10X) 2 × 10 mL
pNPP Tablets 5 ea
His-AP Tracer 100 dtn
His EIA Monoclonal Antibody 100 dtn
His-Protein EIA Standard 1 ea
AP Wash Buffer Concentrate (150X) 5 mL
10012445-480well
Precoated (Goat Anti-Mouse IgG) EIA 96-Well Strip Plate 5 ea
DEA Buffer Concentrate (10X) 12.5 mL
TBS Assay Buffer Concentrate (10X) 4 × 10 mL
pNPP Tablets 25 ea
His-AP Tracer 500 dtn
His EIA Monoclonal Antibody 500 dtn
His-Protein EIA Standard 1 ea
AP Wash Buffer Concentrate (150X) 12.5 mL
10012445-480SOLID
Precoated (Goat Anti-Mouse IgG) EIA 96-Well Solid Plate 5 ea
DEA Buffer Concentrate (10X) 12.5 mL
TBS Assay Buffer Concentrate (10X) 4 × 10 mL
pNPP Tablets 25 ea
His-AP Tracer 500 dtn
His EIA Monoclonal Antibody 500 dtn
His-Protein EIA Standard 1 ea
AP Wash Buffer Concentrate (150X) 12.5 mL
10012445-96SOLID
Precoated (Goat Anti-Mouse IgG) EIA 96-Well Solid Plate 1 ea
DEA Buffer Concentrate (10X) 2.5 mL
TBS Assay Buffer Concentrate (10X) 2 × 10 mL
pNPP Tablets 5 ea
His-AP Tracer 100 dtn
His EIA Monoclonal Antibody 100 dtn
His-Protein EIA Standard 1 ea
AP Wash Buffer Concentrate (150X) 5 mL
Size Price Quantity Subtotal
96 solid wells $178.00 $0.00
96 strip wells $178.00 $0.00
480 solid wells $668.00 $0.00
480 strip wells $668.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

  • All 480 well kits are supplied with a single 500 dtn vial of tracer and antiserum that must be used within the stated time following reconstitution.
  • Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay.

Pricing updated 2012-05-25. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample His-Express Detection EIA Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

Related Products

Protein Determination Kit
UltraPure Water

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Cayman Chemical is a manufacturer, supplier and vendor of biochemical reagents, assay kits, antibodies, and proteins.

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