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Steatosis Colorimetric Assay Kit Exclusive

Cayman Chemical Item Number 10012643

Steatosis Colorimetric Assay Kit

See more	Cell-Based Assay (53) Assay Kits (331) Drug Discovery (78) Toxicology (28)

Description

Steatosis, also known as fatty liver, is a pathological process characterized by abnormal accumulation of lipid within cells. While simple steatosis may not be associated with significant impairment of liver function, excessive fat accumulation can lead to cirrhosis and even liver failure. The molecular mechanisms involved in non-alcoholic steatosis are poorly understood and little information is available on the pathway(s) responsible for progressive hepatocellular damage following lipid accumulation. Determining hepatotoxicity is an essential component of the drug discovery process and includes steatosis as one of the key parameters evaluated. Cayman Chemical’s Steatosis Colorimetric Assay Kit provides a convenient tool for evaluating steatosis risk of drug candidates. In this assay, Oil Red O is used to stain neutral lipids in hepatocytes. Lipid accumulation is then quantified using a plate reader after the dye is extracted from the lipid droplets. Chloroquine is included in the kit as a positive control.

Stability	1 year
Storage	4°C
Shipping	Wet ice in continental US; may vary elsewhere

Background Reading

. Non-clinical guideline on drug-induced hepatotoxicity. (2006).

Adams, L.A., Angulo, P., and Lindor, K.D. Nonalcoholic fatty liver disease. CMAJ 172(7) 899-905 (2005).

Donohue, T.M. Alcohol-induced steatosis in liver cells. World J Gastroenterol 13(37) 4974-4978 (2007).

10012643-1ea
Lipid Droplets Assay Fixative (10X)	1 ea
Lipid Droplets Assay Wash Solution	2 × 1 ea
Lipid Droplets Assay Oil Red O Solution	1 ea
Lipid Droplets Assay Dye Extraction Solution	1 ea
Chloroquine Positive Control (25 mM)	1 ea
Steatosis Assay Hematoxylin	1 ea

Size	Price	Quantity	Subtotal
1 ea	$153.00		$0.00
Bulk	Contact
		Cart Total	$0.00

Pricing updated 2012-05-25. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Steatosis Colorimetric Assay Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

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Downloads

	Download Kit Booklet 507 Kb PDF
	Download Material Safety Data Sheet (MSDS) 86 Kb PDF
	Printer-friendly version of this page small PDF (usually less than 100 Kb)
	• US Letter paper 8½″ × 11″
	• A4 paper 21cm × 29.7cm
	Chemical structure definitions are available for many Cayman products. See Chemical Structure Database for details.

Batch-specific Information

Cayman Chemical is a manufacturer, supplier and vendor of biochemical reagents, assay kits, antibodies, and proteins.

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