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Biosynthesis of 20-HETE from arachidonic acid by the cytochrome P450 4A (CYP450 4A) isoforms is an important component of vascular homeostasis, especially in renal circulation.1 DDMS is a mechanism-based, irreversible inhibitor that has about 10-fold selectivity for the CYP4A2 enzyme which predominantly synthesizes 20-HETE in the mammalian kidney. DDMS administration in whole anesthetized rats (10 mg/kg) largely ablates the hypotension and vasodilation induced by nitric oxide donors such as NONOates.2
1
Lasker, J.M., Chen, W.B., Wolf, I., et al. Formation of 20-hydroxyeicosatetraenoic acid, a vasoactive and natriuretic eicosanoid, in human kidney. Role of CYP4F2 and CYP4A11. J Biol Chem275(6)4118-4126(2000).
2
Alonso-Galicia, M., Drummond, H.A., Reddy, K.K., et al. Inhibition of 20-HETE production contributes to the vascular responses to nitric oxide. Hypertension29320-325(1997).
Room temperature
in continental US; may vary elsewhere
SMILES
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BrC(=CCCCCCCCCCC(=O)NS(=O)(=O)C)Br
Background Reading
Alonso-Galicia, M., Drummond, H.A., Reddy, K.K., et al. Inhibition of 20-HETE production contributes to the vascular responses to nitric oxide. Hypertension29320-325(1997).
Lasker, J.M., Chen, W.B., Wolf, I., et al. Formation of 20-hydroxyeicosatetraenoic acid, a vasoactive and natriuretic eicosanoid, in human kidney. Role of CYP4F2 and CYP4A11. J Biol Chem275(6)4118-4126(2000).
DDMS is available in the following screening
library: