Antigen: human, rat, and mouse CB₁ receptor sequence amino acids 1-14 (MKSILDGLADTTFR) · Host: rabbit · Application(s): WB (does not work for IHC-frozen-tissue-sections); other applications not tested · The CB₁ receptor and the splice variant CB1a are localized mainly in the brain.^1,2,3 · The CB₁ receptor is a G-protein coupled receptor that binds the active component of cannabis, Δ⁹-tetrahydrocannabinol. This antisera has been raised against the N-terminal (amino acids 1-14) extracellular region of the of the CB₁ receptor and can be used for Western blotting.^4,5 Human and rat CB₁ receptors exhibit 97.3% homology at the amino acid level over the complete protein, and 100% homology within the peptide sequence used to make this antibody.^6,1 This peptide exhibits no homology with the CB₂ receptor. Based on the amino acid sequence, the CB₁ receptor has a molecular weight of approximately 52,800.¹ The CB₁ receptor and the splice variant CB1a are localized mainly in the brain, whereas the CB₂ receptor is localized predominantly in peripheral tissues, including the spleen and hemopoietic cells.^6,1,2,3

1 Matsuda, L.A., Lolait, S.J., Brownstein, M.J., et al. Structure of a cannabinoid receptor and functional expression of the cloned cDNA. Nature 346 561-564 (1990).

2 Shire, D., Carillon, C., Kaghad, M., et al. An amino-terminal variant of the central cannabinoid receptor resulting from alternative splicing. J Biol Chem 270 3726-3731 (1995).

3 Munro, S., Thomas, K.L., and Abu-Shaar, M. Molecular characterization of a peripheral receptor for cannabinoids. Nature 365 61-65 (1993).

4 Howlett, A.C., Song, C., Berglund, B.A., et al. Characterization of CB₁ cannabinoid receptors using receptor peptide fragments and site-directed antibodies. Mol Pharmacol 53 504-510 (1998).

5 McIntosh, H.H., Song, C., and Howlett, A.C. CB₁ cannabinoid receptor: Cellular regulation and distribution in N18TG2 neuroblastoma cells. Mol Brain Res 53 163-173 (1998).

6 Gérard, C.M., Mollereau, C., Vassart, G., et al. Molecular cloning of a human cannabinoid receptor which is also expressed in testis. Biochem J 279 129-134 (1991).

Matsuda, L.A., Lolait, S.J., Brownstein, M.J., et al. Structure of a cannabinoid receptor and functional expression of the cloned cDNA. Nature 346 561-564 (1990).

Shire, D., Carillon, C., Kaghad, M., et al. An amino-terminal variant of the central cannabinoid receptor resulting from alternative splicing. J Biol Chem 270 3726-3731 (1995).

Howlett, A.C., Song, C., Berglund, B.A., et al. Characterization of CB₁ cannabinoid receptors using receptor peptide fragments and site-directed antibodies. Mol Pharmacol 53 504-510 (1998).

McIntosh, H.H., Song, C., and Howlett, A.C. CB₁ cannabinoid receptor: Cellular regulation and distribution in N18TG2 neuroblastoma cells. Mol Brain Res 53 163-173 (1998).

Gérard, C.M., Mollereau, C., Vassart, G., et al. Molecular cloning of a human cannabinoid receptor which is also expressed in testis. Biochem J 279 129-134 (1991).

Munro, S., Thomas, K.L., and Abu-Shaar, M. Molecular characterization of a peripheral receptor for cannabinoids. Nature 365 61-65 (1993).

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