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Antigen:
human, rat, and murine CB1 receptor sequence amino acids 1-14 (MKSILDGLADTTFR)
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Host:
rabbit
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Application(s):
WB (does not work for IHC-frozen-tissue-sections); other applications not tested
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The CB1 receptor and the splice variant CB1a are localized mainly in the brain.1,2,3 · The CB1 receptor is a G-protein coupled receptor that binds the active component of cannabis, Δ9-tetrahydrocannabinol. This antisera has been raised against the N-terminal (amino acids 1-14) extracellular region of the of the CB1 receptor and can be used for Western blotting.4,5 Human and rat CB1 receptors exhibit 97.3% homology at the amino acid level over the complete protein, and 100% homology within the peptide sequence used to make this antibody.6,1 This peptide exhibits no homology with the CB2 receptor. Based on the amino acid sequence, the CB1 receptor has a molecular weight of approximately 52,800.1 The CB1 receptor and the splice variant CB1a are localized mainly in the brain, whereas the CB2 receptor is localized predominantly in peripheral tissues, including the spleen and hemopoietic cells.6,1,2,3
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1
Matsuda, L.A., Lolait, S.J., Brownstein, M.J., et al. Structure of a cannabinoid receptor and functional expression of the cloned cDNA. Nature 346 561-564 (1990).
2
Shire, D., Carillon, C., Kaghad, M., et al. An amino-terminal variant of the central cannabinoid receptor resulting from alternative splicing. J Biol Chem 270 3726-3731 (1995).
3
Munro, S., Thomas, K.L., Abu-Shaar, M. Molecular characterization of a peripheral receptor for cannabinoids. Nature 365 61-65 (1993).
4
Howlett, A.C., Song, C., Berglund, B.A., et al. Characterization of CB1 cannabinoid receptors using receptor peptide fragments and site-directed antibodies. Mol Pharmacol 53 504-510 (1998).
5
McIntosh, H.H., Song, C., Howlett, A.C. CB1 cannabinoid receptor: Cellular regulation and distribution in N18TG2 neuroblastoma cells. Mol Brain Res 53 163-173 (1998).
6
Gérard, C.M., Mollereau, C., Vassart, G., et al. Molecular cloning of a human cannabinoid receptor which is also expressed in testis. Biochem J 279 129-134 (1991).
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