Antigen: human CB₂ receptor sequence amino acids 20-33 (NPMKDYMILSGPQK) · Host: rabbit · Application(s): WB and IHC; other applications not tested · The CB₂ receptor is localized predominantly in peripheral tissues, including the spleen and hemopoietic cells.¹ · The CB₁ and CB₂ receptors are G-protein coupled receptors that bind the active component of cannabis, Δ⁹-tetrahydrocannabinol, as well as anandamide which is an endogenous CB receptor ligand. This antibody has been raised against a sequence between the N-terminus and the first transmembrane domain of the protein of the human CB₂ receptor.¹ It can be used for Western blotting and immunohistochemistry applications. Conserved amino acids between the CB₁ and CB₂ receptors in this region are minimal thereby preventing cross-reactivity of this antibody with the CB₁ receptor.² Human and murine CB₂ receptors exhibit 82% homology at the amino acid level over the complete protein.² The CB₂ receptor is localized predominantly in peripheral tissues, including the spleen and hemopoietic cells.¹

1 Munro, S., Thomas, K.L., Abu-Shaar, M. Molecular characterization of a peripheral receptor for cannabinoids. Nature 365 61-65 (1993).

2 Shire, D., Calandra, B., Rinaldi-Carmona, M., et al. Molecular cloning, expression and function of the murine CB₂ peripheral cannabinoid receptor. Biochim Biophys Acta 1307 132-136 (1996).

Munro, S., Thomas, K.L., Abu-Shaar, M. Molecular characterization of a peripheral receptor for cannabinoids. Nature 365 61-65 (1993).

Casanova, M.L., Blázquez, C., Martínez-Palacio, J., et al. Inhibition of skin tumor growth and angiogenesis in vivo by activation of cannabinoid receptors. J Clin Invest 111(1) 43-50 (2003).

Shire, D., Calandra, B., Rinaldi-Carmona, M., et al. Molecular cloning, expression and function of the murine CB₂ peripheral cannabinoid receptor. Biochim Biophys Acta 1307 132-136 (1996).