Antigen: mouse DP₁ receptor N-terminal amino acids 2-21 · Host: rabbit · Application(s): WB and ICC; other applications not tested · The DP₁ receptor is one of two receptor isoforms for prostaglandin D₂ (PGD₂), a major cyclooxygenase metabolite of arachidonic acid. As a G-protein coupled receptor (GPCR), the receptor primarily couples to Gs to increase cAMP, but also transiently increases Ca2+, apparently through the cAMP pathway.¹ DP₁ receptor mRNA has been detected in various tissues and cells including brain, spleen, lung, bone marrow, stomach, skin, and mast cells,^2,3,4 however little is know about its protein localization due to its low level of expression. Cayman’s DP₁ receptor polyclonal antibody detects the protein by western blot in samples such as cerebral cortex, hippocampus, and HT-29 cells, a colorectal cancer cell line shown to express this receptor.⁵ The antibody can also be used in immunocytochemistry/immunohistochemistry to detect DP₁ receptor expression in cells and tissues.

1 Kabashima, K., and Narumiya, S. The DP receptor, allergic inflammation and asthma. Prostaglandins Leukot Essent Fatty Acids 69 187-194 (2003).

2 Hirata, M., Kakizuka, A., Aizawa, M., et al. Molecular characterization of a mouse prostaglandin D receptor and functional expression of the cloned gene. Proc Natl Acad Sci USA 91 11192-11196 (1994).

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4 Narumiya, S., Sugimoto, Y., and Ushikubi, F. Prostanoid receptors: Structures, properties, and functions. Physiol Rev 79 1193-1226 (1999).

5 Hawcroft, G., Gardner, S.H., and Hull, M.A. Expression of prostaglandin D₂ receptors DP₁ and DP₂ by human colorectal cancer cells. Cancer Lett 210 81-84 (2004).

Kabashima, K., and Narumiya, S. The DP receptor, allergic inflammation and asthma. Prostaglandins Leukot Essent Fatty Acids 69 187-194 (2003).

Hirata, M., Kakizuka, A., Aizawa, M., et al. Molecular characterization of a mouse prostaglandin D receptor and functional expression of the cloned gene. Proc Natl Acad Sci USA 91 11192-11196 (1994).

Narumiya, S., Sugimoto, Y., and Ushikubi, F. Prostanoid receptors: Structures, properties, and functions. Physiol Rev 79 1193-1226 (1999).

Hawcroft, G., Gardner, S.H., and Hull, M.A. Expression of prostaglandin D₂ receptors DP₁ and DP₂ by human colorectal cancer cells. Cancer Lett 210 81-84 (2004).

Boie, Y., Sawyer, N., Slipetz, D.M., et al. Molecular cloning and characterization of the human prostanoid DP receptor. J Biol Chem 270 18910-18916 (1995).

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