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Arachidonoyl p-Nitroaniline

Cayman Chemical Item Number 10168

ApNA (CAS 119520-58-0)

Arachidonoyl p-Nitroaniline (CAS 119520-58-0)

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Description

Arachidonoyl p-nitroaniline (ApNA) is one of several nitroaniline fatty acid amides which can be used to measure fatty acid amide hydrolase (FAAH) activity.1 FAAH is a relatively unselective enzyme in that it accepts a variety of amide head groups other than the ethanolamine of its nominal endogenous substrate anandamide (AEA). It also will hydrolyze fatty acid amides with fewer carbons and fewer double bonds than arachidonate. (See also Decanoyl p-Nitroaniline - Catalog No. 90349). Exposure of ApNA to FAAH activity results in the release of the yellow colorimetric dye p-nitroaniline (ε = 13,500 at 382 nm). This offers the potential for fast and convenient measurements of FAAH activity using a 96-well plate spectrophotometer.

1 Patricelli, M.P., and Cravatt, B.F. Characterization and manipulation of the acyl chain selectivity of fatty acid amide hydrolase. Biochemistry 40 6107-6115 (2001).

Synonyms
  • ApNA
Formal Name N-​(4-​nitrophenyl)-​5Z,​8Z,​11Z,​14Z-​eicosatetraenamide
CAS Number 119520-58-0
Molecular Formula C26H36N2O3
Formula Weight 424.6
Formulation A solution in methyl acetate
Purity >98%
λmax 204, 316 nm
Stability 2 years
Storage -20°C
Shipping Room temperature in continental US; may vary elsewhere
SMILES
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CCCCC/C=C\C/C=C\C/C=C\C/C=C\CCCC(=O)​Nc1ccc(cc1)​[N]​(=O)​O

Background Reading

Patricelli, M.P., and Cravatt, B.F. Characterization and manipulation of the acyl chain selectivity of fatty acid amide hydrolase. Biochemistry 40 6107-6115 (2001).

Arachidonoyl p-Nitroaniline is available in the following screening library:

Size Price Quantity Subtotal
1 mg $11.00 $0.00
5 mg $50.00 $0.00
10 mg $88.00 $0.00
50 mg $385.00 $0.00
Bulk Contact
Cart Total $0.00

This product is available in custom sizes and/or larger quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-05-26. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

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