Cayman Chemical | PPARγ Polyclonal Antibody | Description • Peroxisome Proliferator-activated Receptor γ

Antigen: human PPARγ1 amino acids 82-101 (PASPPYYSEKTQLYNKPHEE; amino acids 110-129 of PPARγ2) · Host: rabbit · Application(s): WB; other applications not tested · PPARγ is a ligand-activated transcription factor involved in the regulation of lipid homeostasis and may function as a master regulator of adipogenesis.^1,2,3,4 PPARγ mRNA is expressed prominently in adipose tissue, but is also found in large intestine, kidney, liver, and small intestine.⁵ Alternative splicing of the PPARγ gene results in at least two mRNA species that differ at their 5' ends.^1,6 Human PPARγ1 and PPARγ2 proteins are 53 and 57 kDa, respectively, based on the deduced amino acid sequences.⁶ PPARγ2 is the major PPARγ isoform found in both the cytosolic and nuclear fractions of undifferentiated 3T3-L1 cells.⁷ Total cellular PPARγ2 protein increases approximately 2-fold following differentiation of 3T3-L1 cells, whereas only small quantities of PPARγ1 are detected in the nuclear fraction following differentiation.⁷

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6 Mukherjee, R., Jow, L., Croston, G.E., et al. Identification, characterization, and tissue distribution of human peroxisome proliferator-activated receptor (PPAR) isoforms PPARγ2 versus PPARγ1 and activation with retinoid X receptor agonists and antagonists. J Biol Chem 272 8071-8076 (1997).

7 Thuillier, P., Baillie, R., Sha, X., et al. Cytosolic and nuclear distribution of PPARγ2 in differentiating 3T3-L1 preadipocytes. J Lipid Res 39 2329-2338 (1998).

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