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Antigen:
human, mouse, and rat amino acids 22-36 (PLSEEFLQEMGNIQE)
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Host:
rabbit
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Application(s):
WB; other applications not tested
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PPARα is a ligand-activated transcription factor involved in the regulation of lipid homeostasis.1,2 Fatty acids, NSAIDs, parotaglandins, leukotriene B4 (LTB4), WY 14643, and hypolipidemic drugs are PPARα ligands.3,4,5 PPARα transcriptionally regulates a variety of genes for enzymes and proteins involved in fatty acid metabolism and oxidation, such as acyl-CoA oxidase, enol-CoA hydratase, medium chain fatty acyl-CoA dehydrogenase, fatty acid transport protein, and cytochrome P450 4A isozymes.2,6,7 Northern blot analysis reveals that PPARα is expressed predominately in liver, skeletal muscle, heart, and kidney.8 The molecular weight of PPARα is approximately 52,000 based on the deduced amino acid sequence.
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1
Latruffe, N., Vamecq, J. Peroxisome proliferators and peroxisome proliferator activated receptors (PPARs) as regulators of lipid metabolism. Biochimie 79 81-94 (1997).
2
Lemberger, T., Desvergne, B., Wahli, W. Peroxisome proliferator-activated receptors: A nuclear receptor signaling pathway in lipid physiology. Annu Rev Cell Dev Biol 12 335-363 (1996).
3
Devchand, P.R., Keller, H., Peters, J.M., et al. The PPARα-leukotriene B4 pathway to inflammation control. Nature 384 39-43 (1996).
4
Lehmann, J.M., Lenhard, J.M., Oliver, B.B., et al. Peroxisome proliferator-activated receptors a and g are activated by indomethacin and other non-steroidal anti-inflammatory drugs. J Biol Chem 272 3406-3410 (1997).
5
Forman, B.M., Chen, J., Evans, R.M. Hypolipidemic drugs, polyunsaturated fatty acids, and eicosanoids are ligands for peroxisome proliferator-activated receptors α and δ Proc Natl Acad Sci USA 94, 4312-4317 (1997).
6
Martin, G., Schoonjans, K., Lefevbre, A., et al. Coordinate regulation of the expression of the fatty acid transport protein and acyl-CoA synthetase genes by PPARα and PPARγ activators. J Biol Chem 272 28210-28217 (1997).
7
Kroetz, D.L., Yook, P., Costet, P., et al. Peroxisome proliferator-activated receptor α controls the hepatic CYP4A induction adaptive response to starvation and diabetes. J Biol Chem 273 31581-31589 (1998).
8
Mukherjee, R., Jow, L., Noonan, D., et al. Human and rat peroxisome proliferator activated receptors (PPARs) demonstrate similar tissue distribution but different responsiveness to PPAR activators. J Steroid Biochem Molec Biol 51 157-166 (1994).
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