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Antigen:
human recombinant MCAD
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Host:
rabbit
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Application(s):
WB; other applications not tested
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Medium-chain fatty acyl-CoA dehydrogenase (MCAD) is a mitochondrial enzyme that catalyzes the first step in the βoxidation of fatty acids. MCAD expression is induced during periods of fasting, when reliance on fatty acids for energy is increased.1,2 The promoter for MCAD contains a peroxisome proliferator response element (PPRE) and is regulated transcriptionally by peroxisome proliferator-activated receptor alpha (PPARα), a ligand-activated transcription factor involved in the regulation of lipid homeostasis.1,3,4 Because of this, MCAD expression can be used as a marker to evaluate the in vivo activity of PPARα1,2 Human MCAD is approximately 87% homologous to porcine and rat MCAD, respectively.5,6 MCAD is expressed in liver, heart, kidney, and skeletal muscle.
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1
Gulick, T., Cresci, S., Caira, T., et al. The peroxisome proliferator-activated receptor regulates mitochondrial fatty acid oxidative enzyme gene expression. Proc Natl Acad Sci USA 91 11012-11016 (1994).
2
Leone, T.C., Weinheimer, C.J., Kelly, D.P. A critical role for the peroxisome proliferator-activated receptor α (PPARα) in the cellular fasting response: The PPARα-null mouse as a model of fatty acid oxidation disorders. Proc Natl Acad Sci USA 96 7473-7478 (1999).
3
Carter, M.E., Gulick, T., Moore, D.D., et al. A pleiotropic element in the medium-chain acyl coenzyme A dehydrogenase gene promoter mediates transcriptional regulation by multiple nuclear receptor transcription factors and defines novel receptor-DNA binding motifs. Mol Cell Biol 14 4360-4372 (1994).
4
Lemberger, T., Desvergne, B., Wahli, W. Peroxisome proliferator-activated receptors: A nuclear receptor signaling pathway in lipid physiology. Annu Rev Cell Dev Biol 12 335-363 (1996).
5
Kelly, D.P., Kim, J., Billadello, J.J., et al. Nucleotide sequence of medium-chain acyl-CoA dehydrogenase mRNA and its expression in enzyme-deficient human tissue. Proc Natl Acad Sci USA 84 4068-4072 (1987).
6
Matsubara, Y., Kraus, J.P., Ozasa, H., et al. Molecular cloning and nucleotide sequence of cDNA encoding the entire precursor of rat liver medium chain acyl coenzyme A dehydrogenase. J Biol Chem 262 10104-10108 (1987).
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