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Antigen:
human EP1 receptor C-terminal amino acids 380-402 (GLTPSAWEASSLRSSRHSGLSHF)
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Host:
rabbit
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Application(s):
WB and ICC
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The biological effects of PGE2 are mediated through interaction with four distinct membrane-bound G-protein coupled EP receptors: EP1, EP2, EP3, and EP4.1,2 Binding of PGE2 to the EP1 receptor results in an increase in phosphatidyl inositol turnover with subsequent increase in intracellular free Ca2+.3,4 Pharmacologically, EP1 receptors mediate contraction of smooth muscle.1 The human EP1 receptor is comprised of 402 amino acids with a molecular mass of approximately 42,000.3 The EP1 receptor is expressed in a variety of tissues, including the kidney, lung, and sensory neuron.3,4,5 Within the kidney, the EP1 receptor is expressed at high levels in the cortical, outer medullary, and inner medullary collecting duct.6
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1
Coleman, R.A., Smith, W.L., Narumiya, S. Classification of prostanoid receptors: Properties, distribution, and structure of the receptors and their subtypes. Pharmacol Rev 46 205-229 (1994).
2
Coleman, R.A., Eglen, R.M., Jones, R.L., et al. Classification of prostanoid receptors IUPHAR receptor compendium. IUPHAR Compendium (1997).
3
Funk, C.D., Furci, L., Fitzgerald, G.A., et al. Cloning and expression of a cDNA for the human prostaglandin E receptor EP1 subtype. J Biol Chem 268 26767-26772 (1993).
4
Honda, A., Sugimoto, Y., Namba, T., et al. Cloning and expression of a cDNA for mouse prostaglandin E receptor EP2T subtype. J Biol Chem 268 7759-7762 (1993).
5
Southall, M.D., Vasko, M.R. Prostaglandin receptor subtypes, EP3C and EP4, mediate the prostaglandin E2-induced cAMP production and sensitization of sensory neurons. J Biol Chem 276(19) 16083-16091 (2001).
6
Breyer, M.D., Davis, L., Jacobson, H.R., et al. Differential localization of prostaglandin E receptor subtypes in human kidney. Am J Physiol 270, F912-F918 (1996).
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