Antigen: human EP₁ receptor C-terminal amino acids 380-402 (GLTPSAWEASSLRSSRHSGLSHF) · Host: rabbit · Application(s): WB and ICC · The biological effects of PGE₂ are mediated through interaction with four distinct membrane-bound G-protein coupled EP receptors: EP₁, EP₂, EP₃, and EP₄.^1,2 Binding of PGE₂ to the EP₁ receptor results in an increase in phosphatidyl inositol turnover with subsequent increase in intracellular free Ca2+.^3,4 Pharmacologically, EP₁ receptors mediate contraction of smooth muscle.¹ The human EP₁ receptor is comprised of 402 amino acids with a molecular mass of approximately 42,000.³ The EP₁ receptor is expressed in a variety of tissues, including the kidney, lung, and sensory neuron.^3,4,5 Within the kidney, the EP₁ receptor is expressed at high levels in the cortical, outer medullary, and inner medullary collecting duct.⁶

1 Coleman, R.A., Smith, W.L., and Narumiya, S. Classification of prostanoid receptors: Properties, distribution, and structure of the receptors and their subtypes. Pharmacol Rev 46 205-229 (1994).

2 Coleman, R.A., Eglen, R.M., Jones, R.L., et al. Classification of prostanoid receptors IUPHAR receptor compendium. IUPHAR Compendium 1-12 (1997).

3 Funk, C.D., Furci, L., Fitzgerald, G.A., et al. Cloning and expression of a cDNA for the human prostaglandin E receptor EP₁ subtype. J Biol Chem 268 26767-26772 (1993).

4 Honda, A., Sugimoto, Y., Namba, T., et al. Cloning and expression of a cDNA for mouse prostaglandin E receptor EP2T subtype. J Biol Chem 268 7759-7762 (1993).

5 Southall, M.D., and Vasko, M.R. Prostaglandin receptor subtypes, EP3C and EP₄, mediate the prostaglandin E₂-induced cAMP production and sensitization of sensory neurons. J Biol Chem 276(19) 16083-16091 (2001).

6 Breyer, M.D., Davis, L., Jacobson, H.R., et al. Differential localization of prostaglandin E receptor subtypes in human kidney. Am J Physiol 270 F912-F918 (1996).

Coleman, R.A., Smith, W.L., and Narumiya, S. Classification of prostanoid receptors: Properties, distribution, and structure of the receptors and their subtypes. Pharmacol Rev 46 205-229 (1994).

Funk, C.D., Furci, L., Fitzgerald, G.A., et al. Cloning and expression of a cDNA for the human prostaglandin E receptor EP₁ subtype. J Biol Chem 268 26767-26772 (1993).

Coleman, R.A., Eglen, R.M., Jones, R.L., et al. Classification of prostanoid receptors IUPHAR receptor compendium. IUPHAR Compendium 1-12 (1997).

Cosme, R., Lublin, D., Takafuji, V., et al. Prostanoids in human colonic mucosa: Effects of inflammation on PGE₂ receptor expression . Hum Immunol 61 684-696 (2000).

Southall, M.D., and Vasko, M.R. Prostaglandin receptor subtypes, EP3C and EP₄, mediate the prostaglandin E₂-induced cAMP production and sensitization of sensory neurons. J Biol Chem 276(19) 16083-16091 (2001).

Breyer, M.D., Davis, L., Jacobson, H.R., et al. Differential localization of prostaglandin E receptor subtypes in human kidney. Am J Physiol 270 F912-F918 (1996).

Honda, A., Sugimoto, Y., Namba, T., et al. Cloning and expression of a cDNA for mouse prostaglandin E receptor EP2T subtype. J Biol Chem 268 7759-7762 (1993).

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