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Antigen:
human EP2 receptor C-terminal amino acids 335-358 (SLRTQDATQTSCSTQSDASKQADL)
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Host:
rabbit
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Application(s):
WB and ICC
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The biological effects of prostaglandin E2 (PGE2) are mediated through interaction with four distinct membrane-bound G-protein coupled EP receptors: EP1, EP2, EP3, and EP4.1,2 Binding of PGE2 to the EP2 receptor results in an increase in adenylate cyclase activity with a subsequent increase in cAMP.3,4 Pharmacologicially, EP2 receptors mediate relaxation of smooth muscle and are distinguished from EP4 receptors by their sensitivity to the EP2 receptor selective agonist butaprost.3,1,2 The human EP2 receptor is comprised of 358 amino acids with a molecular mass of approximately 40,000.3 A band at 53 kDa is detected in most samples by Western blotting. mRNA for the EP2 receptor is expressed in a variety of tissues including lung, placenta, spleen, intestine, kidney, and sensory neuron.3,4,5
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1
Coleman, R.A., Smith, W.L., Narumiya, S. Classification of prostanoid receptors: Properties, distribution, and structure of the receptors and their subtypes. Pharmacol Rev 46 205-229 (1994).
2
Coleman, R.A., Dahlén, S., Drazen, J.M., et al. Classification of leukotriene receptors IUPHAR receptor compendium. IUPHAR Compendium (1997).
3
Regan, J.W., Bailey, T.J., Pepperl, D.J., et al. Cloning of a novel human prostaglandin receptor with characteristics of the pharmacologically defined EP2 subtype. Mol Pharmacol 46 213-220 (1994).
4
Nemoto, K., Pilbeam, C.C., Bilak, S.R., et al. Molecular cloning and expression of a rat prostaglandin E2 receptor of the EP2 subtype. Prostaglandins 54 713-725 (1997).
5
Southall, M.D., Vasko, M.R. Prostaglandin receptor subtypes, EP3C and EP4, mediate the prostaglandin E2-induced cAMP production and sensitization of sensory neurons. J Biol Chem 276(19) 16083-16091 (2001).
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