Antigen: human EP₂ receptor C-terminal amino acids 335-358 (SLRTQDATQTSCSTQSDASKQADL) · Host: rabbit · Application(s): WB and ICC · The biological effects of prostaglandin E₂ (PGE₂) are mediated through interaction with four distinct membrane-bound G-protein coupled EP receptors: EP₁, EP₂, EP₃, and EP₄.^1,2 Binding of PGE₂ to the EP₂ receptor results in an increase in adenylate cyclase activity with a subsequent increase in cAMP.^3,4 Pharmacologicially, EP₂ receptors mediate relaxation of smooth muscle and are distinguished from EP₄ receptors by their sensitivity to the EP₂ receptor selective agonist butaprost.^3,1,2 The human EP₂ receptor is comprised of 358 amino acids with a molecular mass of approximately 40,000.³ A band at 53 kDa is detected in most samples by Western blotting. mRNA for the EP₂ receptor is expressed in a variety of tissues including lung, placenta, spleen, intestine, kidney, and sensory neuron.^3,4,5

1 Coleman, R.A., Smith, W.L., and Narumiya, S. Classification of prostanoid receptors: Properties, distribution, and structure of the receptors and their subtypes. Pharmacol Rev 46 205-229 (1994).

2 Coleman, R.A., Dahlén, S., Drazen, J.M., et al. Classification of leukotriene receptors IUPHAR receptor compendium. IUPHAR Compendium 1-7 (1997).

3 Regan, J.W., Bailey, T.J., Pepperl, D.J., et al. Cloning of a novel human prostaglandin receptor with characteristics of the pharmacologically defined EP₂ subtype. Mol Pharmacol 46 213-220 (1994).

4 Nemoto, K., Pilbeam, C.C., Bilak, S.R., et al. Molecular cloning and expression of a rat prostaglandin E₂ receptor of the EP₂ subtype. Prostaglandins 54 713-725 (1997).

5 Southall, M.D., and Vasko, M.R. Prostaglandin receptor subtypes, EP3C and EP₄, mediate the prostaglandin E₂-induced cAMP production and sensitization of sensory neurons. J Biol Chem 276(19) 16083-16091 (2001).

Coleman, R.A., Smith, W.L., and Narumiya, S. Classification of prostanoid receptors: Properties, distribution, and structure of the receptors and their subtypes. Pharmacol Rev 46 205-229 (1994).

Coleman, R.A., Dahlén, S., Drazen, J.M., et al. Classification of leukotriene receptors IUPHAR receptor compendium. IUPHAR Compendium 1-7 (1997).

Southall, M.D., and Vasko, M.R. Prostaglandin receptor subtypes, EP3C and EP₄, mediate the prostaglandin E₂-induced cAMP production and sensitization of sensory neurons. J Biol Chem 276(19) 16083-16091 (2001).

Cosme, R., Lublin, D., Takafuji, V., et al. Prostanoids in human colonic mucosa: Effects of inflammation on PGE₂ receptor expression . Hum Immunol 61 684-696 (2000).

Narko, K., Saukkonen, K., Ketola, I., et al. Regulated expression of prostaglandin E₂ receptors EP₂ and EP₄ in human ovarian granulosa-luteal cells . J Clin Endocrinol Metab 86(4) 1765-1768 (2001).

Nemoto, K., Pilbeam, C.C., Bilak, S.R., et al. Molecular cloning and expression of a rat prostaglandin E₂ receptor of the EP₂ subtype. Prostaglandins 54 713-725 (1997).

Regan, J.W., Bailey, T.J., Pepperl, D.J., et al. Cloning of a novel human prostaglandin receptor with characteristics of the pharmacologically defined EP₂ subtype. Mol Pharmacol 46 213-220 (1994).

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