Antigen: human EP₃ receptor amino acids 308-327 (NQTSVEHCKTHTEKQKECNF) · Host: rabbit · Application(s): WB and ICC · The biological effects of prostaglandin E₂ (PGE₂) are mediated through interaction with four distinct membrane-bound G-protein coupled EP receptors: EP₁, EP₂, EP₃, and EP₄.^1,2 As a result of splice variation, the EP₃ receptor can be expressed as multiple isoforms that differ in the length and sequence of their C-terminal tails.^3,2 The signal transduction mechanism varies depending on the isoform being expressed, implicating the importance of the C-terminal region of the receptor for coupling to G-proteins.^3,2 The EP₃ receptor is expressed in a variety of tissues with highest levels in kidney, pancreas, and uterus.^3,4,5

1 Coleman, R.A., Eglen, R.M., Jones, R.L., et al. Classification of prostanoid receptors IUPHAR receptor compendium. IUPHAR Compendium 1-12 (1997).

2 Narumiya, S., Sugimoto, Y., and Ushikubi, F. Prostanoid receptors: Structures, properties, and functions. Physiol Rev 79 1193-1226 (1999).

3 Kotani, M., Tanaka, I., Ogawa, Y., et al. Molecular cloning and expression of multiple isoforms of human prostaglandin E receptor EP₃ subtype generated by alternative messenger RNA splicing: Multiple second messenger systems and tissue-specific distributions. Mol Pharmacol 48 869-879 (1995).

4 Sugimoto, Y., Namba, T., Honda, A., et al. Cloning and expression of a cDNA for mouse prostaglandin E receptor EP₃ subtype. J Biol Chem 267 6463-6466 (1992).

5 Yang, J., Xia, M., Goetzl, E.J., et al. Cloning and expression of the EP₃-subtype of human receptors for prostaglandin E₂. Biochem Biophys Res Commun 198 999-1006 (1994).

Narumiya, S., Sugimoto, Y., and Ushikubi, F. Prostanoid receptors: Structures, properties, and functions. Physiol Rev 79 1193-1226 (1999).

Cosme, R., Lublin, D., Takafuji, V., et al. Prostanoids in human colonic mucosa: Effects of inflammation on PGE₂ receptor expression . Hum Immunol 61 684-696 (2000).

Kotani, M., Tanaka, I., Ogawa, Y., et al. Molecular cloning and expression of multiple isoforms of human prostaglandin E receptor EP₃ subtype generated by alternative messenger RNA splicing: Multiple second messenger systems and tissue-specific distributions. Mol Pharmacol 48 869-879 (1995).

Kunapuli, S.P., Mao, G.F., Bastepe, M., et al. Cloning and expression of a prostaglandin E receptor EP₃ subtype from human erythroleukaemia cells. Biochem J 298 263-267 (1994).

Sugimoto, Y., Namba, T., Honda, A., et al. Cloning and expression of a cDNA for mouse prostaglandin E receptor EP₃ subtype. J Biol Chem 267 6463-6466 (1992).

Yang, J., Xia, M., Goetzl, E.J., et al. Cloning and expression of the EP₃-subtype of human receptors for prostaglandin E₂. Biochem Biophys Res Commun 198 999-1006 (1994).

Coleman, R.A., Eglen, R.M., Jones, R.L., et al. Classification of prostanoid receptors IUPHAR receptor compendium. IUPHAR Compendium 1-12 (1997).

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