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Antigen:
human amino acids 1-22 (MSTPGVNSSASLSPDRLNSPVTI)
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Host:
rabbit
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Application(s):
WB; other applications not attempted
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The biological effects of prostaglandin E2 (PGE2) are mediated through interaction with four distinct membrane-bound G-protein coupled EP receptors: EP1, EP2, EP3, and EP4.1,2 Binding of PGE2 to the EP4 receptor causes an increase in intracellular cAMP and subsequent relaxation of smooth muscle.3,4,1,2 In addition to sequence differences, EP4 receptors are distinguished from EP2 receptors by their insensitivity to the EP2 receptor selective agonist butaprost.5,6 The EP4 receptor is expressed in multiple human tissues including kidney, small intestine, thymus, ileum, uterus, and lung, as well as in peripheral blood leukocytes.3,4 The receptor is also expressed in cultured human blood cell lines of monocytoid (U937 and THP-1) and lymphoid (MOLT-4, Jurkat, and Raji) origin.4
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1
Bastien, L., Sawyer, N., Grygorczyk, R., et al. Cloning, functional expression, and characterization of the human prostaglandin E2 receptor EP2 subtype. J Biol Chem 269 11873-11877 (1994).
2
Nishigaki, N., Negishi, M., Honda, A., et al. Identification of prostaglandin E receptor 'EP2' cloned from mastocytoma cells as EP4 subtype. FEBS Lett 364 339-341 (1995).
3
An, S., Yang, J., Xia, M., et al. Cloning and expression of the EP2 subtype of human receptors for prostaglandin E2. Biochem Biophys Res Commun 197 263-270 (1993).
4
Mori, K., Tanaka, I., Kotani, M., et al. Gene expression of the human prostaglandin E receptor EP4 subtype: Differential regulation in monocytoid and lymphoid lineage cells by phorbol ester. J Mol Med 74 333-336 (1996).
5
Coleman, R.A., Eglen, R.M., Jones, R.L., et al. Classification of prostanoid receptors IUPHAR receptor compendium. IUPHAR Compendium (1997).
6
Regan, J.W., Bailey, T.J., Pepperl, D.J., et al. Cloning of a novel human prostaglandin receptor with characteristics of the pharmacologically defined EP2 subtype. Mol Pharmacol 46 213-220 (1994).
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