Antigen: mouse TP receptor amino acids 275-289 (VMSFSGQLLRATEHQ) · Host: rabbit · Application(s): WB and IHC (formalin-fixed paraffin-embedded sections) · Thromboxane A₂ (TXA₂) is a potent vasoconstrictor and activator of platelet aggregation. TXA₂ elicits its effects via a 7-transmembrane domain G-protein coupled receptor, the TP receptor.¹ The TP receptor is highly expressed in platelets and is relatively less abundant in tissues such as lung, kidney, brain, spleen, thymus, monocytes, uterus, and placenta.^2,3,4,5 The apparent molecular weight for TP receptors has been reported from 37 kDa to 70 kDa, depending on different degrees of glycosylation.^6,5,7 However, Cayman’s antibody consistently detects the TP receptor at 42 kDa in kidney, Hep2, and Cos-7 cell samples. Cayman’s TP receptor polyclonal antibody can be used for Western blot analysis for both the TPA and TPB splice variants of the receptor on samples of human, mouse, rat, monkey and bovine origin. Use of this antibody for other species and applications has not yet been tested.

1 Ellis, E.F., Oelz, O., Roberts, L.J., et al. Coronary arterial smooth muscle contraction by a substance released from platelets: Evidence that it is thromboxane A₂. Science 193 1135-1137 (1976).

2 Namba, T., Sugimoto, Y., Hirata, M., et al. Mouse thromboxane A₂ receptor: cDNA cloning, expression and Northern blot analysis. Biochem Biophys Res Commun 184 1197-1203 (1992).

3 Båtshake, B., Nilsson, C., and Sundelin, J. Structure and expression of the murine thromboxane A₂ receptor gene. Biochem Biophys Res Commun 256 391-397 (1999).

4 Mais, D.E., True, T.A., and Martinelli, M.J. Characterization by photoaffinity labelling of the human platelet thromboxane A₂/prostaglandin H₂ receptor: Evidence for N-linked glycosylation. Eur J Pharmacol 227 267-274 (1992).

5 Takahashi, N., Takeuchi, K., Abe, T., et al. Immunolocalization of rat thromboxane receptor in the kidney. Endocrinology 137 5170-5173 (1996).

6 Wang, G., Zhu, Y., Halushka, P.V., et al. Mechanism of platelet inhibition by nitric oxide: In vivo phosphorylation of thromboxane receptor by cyclic GMP-dependent protein kinase. Proc Natl Acad Sci USA 95 4888-4893 (1998).

7 Habib, A., Fitzgerald, G.A., and Maclouf, J. Phosphorylation of the thromboxane receptor α, the predominant isoform expressed in human platelets. J Biol Chem 274 2645-2651 (1999).

Cadroy, Y., and Harker, L.A. Platelets, thrombosis, and antithrombotic therapies. 3rd ed. 515-539 (1990).

Båtshake, B., Nilsson, C., and Sundelin, J. Structure and expression of the murine thromboxane A₂ receptor gene. Biochem Biophys Res Commun 256 391-397 (1999).

Dogné, J., de Leval, X., Benoit, P., et al. Therapeutic potential of thromboxane inhibitors in asthma. Expert Opin Investig Drugs 11(2) 1-8 (2003).

Wang, G., Zhu, Y., Halushka, P.V., et al. Mechanism of platelet inhibition by nitric oxide: In vivo phosphorylation of thromboxane receptor by cyclic GMP-dependent protein kinase. Proc Natl Acad Sci USA 95 4888-4893 (1998).

Namba, T., Sugimoto, Y., Hirata, M., et al. Mouse thromboxane A₂ receptor: cDNA cloning, expression and Northern blot analysis. Biochem Biophys Res Commun 184 1197-1203 (1992).

Janssen, L.J., and Tazzeo, T. Involvement of TP and EP₃ receptors in vasoconstrictor responses to isoprostanes in pulmonary vasculature. J Pharmacol Exp Ther 301(3) 1060-1066 (2002).

Takahashi, N., Takeuchi, K., Abe, T., et al. Immunolocalization of rat thromboxane receptor in the kidney. Endocrinology 137 5170-5173 (1996).

Ellis, E.F., Oelz, O., Roberts, L.J., et al. Coronary arterial smooth muscle contraction by a substance released from platelets: Evidence that it is thromboxane A₂. Science 193 1135-1137 (1976).

Armstrong, R.A. Platelet Prostanoid Receptors. Pharmacotherapy 72 171-191 (1996).

Habib, A., Fitzgerald, G.A., and Maclouf, J. Phosphorylation of the thromboxane receptor α, the predominant isoform expressed in human platelets. J Biol Chem 274 2645-2651 (1999).

Mais, D.E., True, T.A., and Martinelli, M.J. Characterization by photoaffinity labelling of the human platelet thromboxane A₂/prostaglandin H₂ receptor: Evidence for N-linked glycosylation. Eur J Pharmacol 227 267-274 (1992).

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