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Antigen:
human GPR55 amino acids 207-219 (ILLGRRDHTQDWV)
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Host:
rabbit
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Application(s):
WB, FC, and ICC
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GPR55 is a G protein-coupled receptor that has been identified as a novel cannabinoid receptor.1,2 Previously central cannabinoid (CB1) and peripheral cannabinoid (CB2), two widely characterized receptors have been shown to bind THC the active component of cannabis and other endocannabinoids. Mounting evidence suggests that additional receptors play a role in cannabinoid-related signal transduction and GPR55 has been identified as one of them.3 This receptor is widely expressed in the brain, specifically found in large dorsal root ganglion neurons.4 Along with binding THC, it also shows high affinity to lysophosphatidylinositol, anandamide, methanandamide, JWH015, and many other cannabinoid ligands.5,6 The human protein shows 75% and 78% overall sequence homology with the rat and murine proteins, respectively.1 Cayman’s GPR55 Polyclonal Antibody was raised against a human GPR55 synthetic peptide conjugated to KLH. GPR55 is composed of 319 amino acids and has an expected molecular weight of 37 kDa. Post-translational modifications such as glycosylation may retard receptor electrophoretic migration and thereby protein signal may be detected above 37 kDa.
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1
Ryberg, E., Larsson, N., Sjögren, S., et al. The orphan receptor GPR55 is a novel cannabinoid receptor. Br J Pharmacol 152 1092-1101 (2007).
2
Baker, D., Pryce, G., Davies, W.L., et al. In silico patent searching reveals a new cannabinoid receptor. Trends Pharmacol Sci 27(1), 1-4 (2006 Jan).
3
Brown, A.J. Novel cannabinoid receptors. Br J Pharmacol 152 567-575 (2007).
4
Lauckner, J.E., Jensen, J.B., Chen, H., et al. GPR55 is a cannabinoid receptor that increases intracellular calcium and inhibits M current. Proc Natl Acad Sci USA 105(7) 2699-2704 (2008).
5
Oka, S., Nakajima, K., Yamashita, A., et al. Identification of GPR55 as a lysophosphatidylinositol receptor. Biochem Biophys Res Commun 362 928-934 (2007).
6
Zhao, J., Wang, J., Chen, Y., et al. Anti-tumor-promoting activity of a polyphenolic fraction isolated from grape seeds in the mouse skin two-stage initiation-promotion protocol and identification of procyanidin B5-3'-gallate as the most effective antioxidant constituent. Carcinogenesis 20 1737-1745 (1999).
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