Antigen: human GPR55 amino acids 207-219 (ILLGRRDHTQDWV) · Host: rabbit · Application(s): FC, ICC, and WB · GPR55 is a G protein-coupled receptor that has been identified as a novel cannabinoid receptor, although its exact role is still controversial.^1,2 Previously central cannabinoid (CB₁) and peripheral cannabinoid (CB₂), two widely characterized receptors have been shown to bind THC the active component of cannabis and other endocannabinoids. Mounting evidence suggests that additional receptors play a role in cannabinoid-related signal transduction and GPR55 has been identified as one of them.³ This receptor is widely expressed in the brain, specifically found in large dorsal root ganglion neurons.⁴ Along with binding THC, it also shows high affinity to anandamide, methanandamide, JWH015, and many other cannabinoid ligands.^5,6 The human protein shows 75% and 78% overall sequence homology with the rat and murine proteins, respectively.¹ GPR55 is composed of 319 amino acids and has an expected molecular weight of 37 kDa. Post-translational modifications such as glycosylation may retard receptor electrophoretic migration and thereby protein signal may be detected above 37 kDa.

1 Ryberg, E., Larsson, N., Sjögren, S., et al. The orphan receptor GPR55 is a novel cannabinoid receptor. Br J Pharmacol 152 1092-1101 (2007).

2 Baker, D., Pryce, G., Davies, W.L., et al. In silico patent searching reveals a new cannabinoid receptor. Trends Pharmacol Sci 27(1), 1-4 (2006 Jan).

3 Brown, A.J. Novel cannabinoid receptors. Br J Pharmacol 152 567-575 (2007).

4 Lauckner, J.E., Jensen, J.B., Chen, H., et al. GPR55 is a cannabinoid receptor that increases intracellular calcium and inhibits M current. Proc Natl Acad Sci USA 105(7) 2699-2704 (2008).

5 Oka, S., Nakajima, K., Yamashita, A., et al. Identification of GPR55 as a lysophosphatidylinositol receptor. Biochem Biophys Res Commun 362 928-934 (2007).

6 Zhao, J., Wang, J., Chen, Y., et al. Anti-tumor-promoting activity of a polyphenolic fraction isolated from grape seeds in the mouse skin two-stage initiation-promotion protocol and identification of procyanidin B5-3'-gallate as the most effective antioxidant constituent. Carcinogenesis 20 1737-1745 (1999).

Brown, A.J. Novel cannabinoid receptors. Br J Pharmacol 152 567-575 (2007).

Ryberg, E., Larsson, N., Sjögren, S., et al. The orphan receptor GPR55 is a novel cannabinoid receptor. Br J Pharmacol 152 1092-1101 (2007).

Lauckner, J.E., Jensen, J.B., Chen, H., et al. GPR55 is a cannabinoid receptor that increases intracellular calcium and inhibits M current. Proc Natl Acad Sci USA 105(7) 2699-2704 (2008).

Pertwee, R.G. GPR55: A new member of the cannabinoid receptor clan?. Br J Pharmacol 152 984-986 (2007).

Baker, D., Pryce, G., Davies, W.L., et al. In silico patent searching reveals a new cannabinoid receptor. Trends Pharmacol Sci 27(1), 1-4 (2006 Jan).

Oka, S., Nakajima, K., Yamashita, A., et al. Identification of GPR55 as a lysophosphatidylinositol receptor. Biochem Biophys Res Commun 362 928-934 (2007).

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