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Join us! · InformexUSA 2012 · New Orleans, Louisiana · February 14-17, 2012 · Booth 2514

BACE (human recombinant)

Cayman Chemical Item Number 10227

Memapsin-2; β-Secretase; ASP-2; BACE-1; Membrane-bound Aspartic Protease

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Description

Source: recombinant C-terminal hexahistidine-tag protein purified from E. coli · Mr: 48 kDa · Accumulation of the β-amyloid peptide (Aβ) in the brain is implicated as the primary cause of neurodegeneration and progression of Alzheimer’s disease (AD).1 The β-amyloid peptide is derived from sequential proteolytic cleavage of the amyloid precursor protein (APP) by β- and γ-secretases. Initial cleavage by β-secretase (BACE), a membrane-anchored aspartic protease, generates a soluble N-terminal fragment and a membrane-associated C-terminal fragment.2 The C-terminal fragment then undergoes proteolysis by γ-secretase to give the Aβ peptide. BACE has been shown to be the major β-secretase and a promising target as it initiates the first step in Aβ production.1 Inhibition of BACE activity could potentially block the entire cascade of AD pathogenesis. In addition, BACE deficient mice do not generate Aβ peptide. In transgenic murine models of AD driven by Aβ overproduction, BACE deficiency rescued memory deficits and cholinergic dysfunction.3 Additionally, the fact that β-secretase is an aspartic protease has also raised the hope that its therapeutic inhibitor can be as successful as that against HIV protease.

1 Hong, L., Turner, R.T.I., Koelsch, G., et al. Memapsin 2 (β-secretase) as a therapeutic target. Biochem Soc Trans 30(4) 530-534 (2002).

2 Grüninger-Leitch, F., Schlatter, D., Küng, E., et al. Substrate and inhibitor profile of BACE (β-secretase) and comparison with other mammalian aspartic proteases. J Biol Chem 277(7) 4687-4693 (2002).

3 Pietrak, B.L., Crouthamel, M., Tugusheva, K., et al. Biochemical and cell-based assays for characterization of BACE-1 inhibitors. Anal Biochem 342 144-151 (2005).

Synonyms
  • Memapsin-2
  • β-Secretase
  • ASP-2
  • BACE-1
  • Membrane-bound Aspartic Protease
Formulation 100 mM sodium borate, pH 8.5, containing 200 mM sodium chloride
Purity ≥95%
Stability 6 months
Storage -80°C
Shipping Dry ice in continental US; may vary elsewhere

Background Reading

Hong, L., Turner, R.T.I., Koelsch, G., et al. Memapsin 2 (β-secretase) as a therapeutic target. Biochem Soc Trans 30(4) 530-534 (2002).

Pietrak, B.L., Crouthamel, M., Tugusheva, K., et al. Biochemical and cell-based assays for characterization of BACE-1 inhibitors. Anal Biochem 342 144-151 (2005).

Grüninger-Leitch, F., Schlatter, D., Küng, E., et al. Substrate and inhibitor profile of BACE (β-secretase) and comparison with other mammalian aspartic proteases. J Biol Chem 277(7) 4687-4693 (2002).
Size Price Quantity Subtotal
10 µg $56.00 $0.00
25 µg $133.00 $0.00
50 µg $252.00 $0.00
Bulk Contact
Cart Total $0.00

This product is available in custom sizes and/or larger quantities.

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Pricing updated 2012-02-11. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

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