CD3 Monoclonal FITC Antibody (Clone RIV9)

Cayman Chemical Item Number 10231

Description

Clone designation: clone RIV9 · Host: mouse · Application(s): (+) FC, IF, and IHC (frozen sections); (−) WB and IHC (paraffin embedded-sections) · The CD3 antigen is a specific T-cell marker expressed on thymocytes and mature T-lymphocytes. The CD3 complex contains CD3g (M_r = 26 kDa), CD3d (M_r = 21 kDa), and CD3e (M_r = 20 kDa).¹ CD3 is detected on the surface membrane or in the cytoplasm of T-cells from peripheral blood, depending on the state of cell differentiation. In addition to detection of T-cells from peripheral blood, the CD3 antibody can be used for detection of reactive T-cells in lymphoid infiltrates in tissues and neoplastic T-cells in non-Hodgkin’s lymphomas of T-cell lineage.^2,3

1 Berkhout, B., Alarcon, B., and Terhorst, C. Transfection of genes encoding the T cell receptor-associated CD3 complex into COS cells results in assembly of the macromolecular structure. J Biol Chem 263(17) 8528-8536 (1988).

2 Ceuppens, J.L., and Van Vaeck, F. Human T cell activation induced by a monoclonal mouse IgG₃ anti-CD3 antibody (RIV9) requires binding of the Fc part of the antibody to the monocytic 72-kDa high-affinity Fc receptor (FcRI)1. Cell Immunol 118 136-146 (1989).

3 Jiskoot, W., Hoven, A.V., De Koning, A.A.M., et al. Purification and stabilisation of a poorly soluble mouse IgG₃ monoclonal antibody. J Immunol Methods 138 181-189 (1991).

Formulation

100 µg of fluorescein-labeled IgG3 in 500 µl PBS, pH 7.2, containing 50% glycerol, 0.1% BSA, and 0.02% sodium azide

Stability

1 year

Storage

-20°C

Shipping

Wet ice in continental US; may vary elsewhere

Specificity

Human

Background Reading

Ceuppens, J.L., and Van Vaeck, F. Human T cell activation induced by a monoclonal mouse IgG₃ anti-CD3 antibody (RIV9) requires binding of the Fc part of the antibody to the monocytic 72-kDa high-affinity Fc receptor (FcRI)1. Cell Immunol 118 136-146 (1989).

Berkhout, B., Alarcon, B., and Terhorst, C. Transfection of genes encoding the T cell receptor-associated CD3 complex into COS cells results in assembly of the macromolecular structure. J Biol Chem 263(17) 8528-8536 (1988).

Jiskoot, W., Hoven, A.V., De Koning, A.A.M., et al. Purification and stabilisation of a poorly soluble mouse IgG₃ monoclonal antibody. J Immunol Methods 138 181-189 (1991).