Source: human recombinant N-terminal hexahistidine tagged enzyme expressed in E. coli; BC048134 · M_r: 94 kDa · LSD1 belongs to the family of flavin adenine dinucleotide (FAD)-dependent amine oxidases that include monoamine oxidases (MAOs) and polyamine oxidase (PAO).¹ LSD1 is a component of several histone deacetylase co-repressor complexes, including histone deacetylases 1 and 2, CtBP, and the neuronal CoREST complexes.² LSD1, with the help of its cofactor CoREST, specifically demethylates mono- and dimethylated histone H3 lysine 4 (H3-K₄), resulting in transcriptional repression.³ In addition to demethylating histones, LSD1 controls the tumor suppressor activity of p53 by demethylating a specific p53 lysine residue (LYS³⁷⁰). ⁴ This activity does not seem to require CoREST and p53 demethylation of LYS³⁷⁰ prevents p53 interaction with its co-activator 53BP1 to induce apoptosis. ^4,5,6,7

1 Shi, Y., Lan, F., Matson, C., et al. Histone demethylation mediated by the nuclear amine oxidase homolog LSD1. Cell 119 941-953 (2004).

2 Shi, Y., Matson, C., Lan, F., et al. Regulation of LSD1 histone demethylase activity by its associated factors. Mol Cell 19 864 (2005).

3 Forneris, F., Binda, C., Vanoni, M.A., et al. Human histone demethylase LSD1 reads the histone code. J Biol Chem 280(50) 41360-41365 (2005).

4 Huang, J., Sengupta, R., Espejo, A.B., et al. p53 is regulated by the lysine demethylase LSD1. Nature 449 105-108 (2007).

5 Scoumanne, A., and Chen, X. The lysine-specific demethylase 1 is required for cell proliferation in both p53-dependent and -independent manners. J Biol Chem 282(21) 15471-15475 (2007).

6 Tsai, W., Nguyen, T.T., Shi, Y., et al. p53-targeted LSD1 functions in repression of chromatin structure and transcription in vivo. Mol Cell Biol 28(17) 5139-5146 (2008).

7 Scoumanne, A., and Chen, X. Protein methylation: A new mechanism of p53 tumor suppressor regulation. Histol Histopathol 23 1143-1149 (2008).

Tsai, W., Nguyen, T.T., Shi, Y., et al. p53-targeted LSD1 functions in repression of chromatin structure and transcription in vivo. Mol Cell Biol 28(17) 5139-5146 (2008).

Shi, Y., Matson, C., Lan, F., et al. Regulation of LSD1 histone demethylase activity by its associated factors. Mol Cell 19 864 (2005).

Forneris, F., Binda, C., Vanoni, M.A., et al. Human histone demethylase LSD1 reads the histone code. J Biol Chem 280(50) 41360-41365 (2005).

Huang, J., Sengupta, R., Espejo, A.B., et al. p53 is regulated by the lysine demethylase LSD1. Nature 449 105-108 (2007).

Scoumanne, A., and Chen, X. The lysine-specific demethylase 1 is required for cell proliferation in both p53-dependent and -independent manners. J Biol Chem 282(21) 15471-15475 (2007).

Shi, Y., Lan, F., Matson, C., et al. Histone demethylation mediated by the nuclear amine oxidase homolog LSD1. Cell 119 941-953 (2004).

Scoumanne, A., and Chen, X. Protein methylation: A new mechanism of p53 tumor suppressor regulation. Histol Histopathol 23 1143-1149 (2008).

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