PAF Acetylhydrolase (human recombinant)

Cayman Chemical Item Number 10279

PAF-AH; Platelet-Activating Factor Acetylhydrolase; Lp-PLA₂; Lipoprotein PLA₂

Description

Source: active recombinant N-terminal His-tagged protein purified from E. coli · M_r: 46.4 kDa · Platelet-activating factor (PAF) is an important lipid mediator involved in inflammation. Platelet-activating factor acetylhydrolase (PAF-AH) is an extracellular phospholipase A₂ which hydrolyzes the acetyl group at the sn-2 position of phospholipids.¹ Two main types of PAF-AH have been characterized, namely the secreted (i.e. plasma) and intracellular enzymes, which are encoded on individual genes and share only 41% homology at the amino acid level.^2,3 PAF deacetylation by PAF-AH causes loss of biological activity, making PAF-AH potential useful as an anti-inflammation therapy. Human recombinant PAF-AH was characterized using 2-Thio PAF as a substrate, which upon hydrolysis by PAF-AH releases a free thiol that reacts with 5,5-dithio-bis(2-dinitrobenzoic acid) (DTNB) to produce 5-thio-2-nitrobenzoic acid (TNB) with an absorbance maximum at 412 nm.

1 Tjoelker, L.W., Wilder, C., Eberhardt, C., et al. Anti-inflammatory properties of a platelet-activating factor acetylhydrolase. Nature 374 549-553 (1995).

2 Stafforini, D.M., McIntyre, T.M., Zimmerman, G.A., et al. Platelet-activating factor acetylhydrolases. J Biol Chem 272 17895-17898 (1997).

3 missing reference text

Synonyms

PAF-AH
Platelet-Activating Factor Acetylhydrolase
Lp-PLA2
Lipoprotein PLA2

Formulation

100 mM Sodium phosphate, pH 7.2, containing 100 mM sodium chloride and 20% glycerol

Purity

≥95%

Stability

1 year

Storage

-80°C

Shipping

Dry ice in continental US; may vary elsewhere

Background Reading

Tjoelker, L.W., Wilder, C., Eberhardt, C., et al. Anti-inflammatory properties of a platelet-activating factor acetylhydrolase. Nature 374 549-553 (1995).

Stafforini, D.M., McIntyre, T.M., Zimmerman, G.A., et al. Platelet-activating factor acetylhydrolases. J Biol Chem 272 17895-17898 (1997).

Hattori, K., Adachi, H., Matsuzawa, A., et al. cDNA cloning and expression of intracellular platelet-activating factor (PAF) acetylhydrolase II. Its homology with plasma PAF acetylhydrolase. J Biol Chem 271 33032-33038 (1996).

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25 µg 
$153.00

$0.00

50 µg 
$291.00

$0.00

100 µg 
$551.00

$0.00

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PAF Acetylhydrolase (human recombinant)