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NAPE-PLD Blocking Peptide (aa 159-172)

Cayman Chemical Item Number 10303

See more	Antibodies (630)

Description

To be used in conjunction with Cayman’s NAPE-PLD polyclonal antibody (aa 159-172) (Catalog No. 10305) to block protein-antibody complex formation during analysis of NAPE-PLD · N-acylethanolamines (NAEs) are involved in diverse biological processes such as inflammatory regulation, apoptosis, and tissue degeneration.¹ In animals, NAEs are mainly biosynthesized via a membrane phospholipid-dependent pathway, which is the enzymatic hydrolysis of N-acyl-phosphatidylethanolamine (NAPE). The enzyme catalyzing this reaction is a phospholipase D (PLD) subtype selective for NAPE named N-acylphosphatidylethanolamine-hydrolysing PLD (NAPE-PLD). It has been cloned from mouse, rat, and human and is 393-396 amino acids in length, with an estimated molecular weight of 46 kDa. Both NAPE-PLD mRNA and protein activity have been detected in a wide range of tissues with the highest levels in brain, kidney, and testis.² In rat, NAPE-PLD activity in the brain is low in neonates and is 15-fold higher in adults, whereas the activity remains constant in the heart during development.³

1 Hansen, H.S., Moesgaard, B., Petersen, G., et al. Putative neuroprotective actions of N-acyl-ethanolamines. Pharmacol Ther 95 119-126 (2002).

2 Okamoto, Y., Morishita, J., Tsuboi, K., et al. Molecular characterization of a phospholipase D generating anandamide and its congeners. J Biol Chem 279(7) 5298-5305 (2004).

3 Moesgaard, B., Petersen, G., Jaroszewski, J.W., et al. Age dependent accumulation of N-acyl-ethanolamine phospholipids in ischemic rat brain: A 31P NMR and enzyme activity study. J Lipid Res 41 985-990 (2000 Jan 1).

Formulation	200 µg of peptide lyophilized from from 200 µl water
Stability	1 year
Storage	-20°C
Shipping	Wet ice in continental US; may vary elsewhere

Background Reading

Okamoto, Y., Morishita, J., Tsuboi, K., et al. Molecular characterization of a phospholipase D generating anandamide and its congeners. J Biol Chem 279(7) 5298-5305 (2004).

Hansen, H.S., Moesgaard, B., Petersen, G., et al. Putative neuroprotective actions of N-acyl-ethanolamines. Pharmacol Ther 95 119-126 (2002).

Moesgaard, B., Petersen, G., Jaroszewski, J.W., et al. Age dependent accumulation of N-acyl-ethanolamine phospholipids in ischemic rat brain: A 31P NMR and enzyme activity study. J Lipid Res 41 985-990 (2000 Jan 1).

Size	Price	Quantity	Subtotal
200 µg	$107.00		$0.00
Bulk	Contact
		Cart Total	$0.00

Pricing updated 2012-02-09. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

Related Products

1-Palmitoyl-2-linoleoyl PE

NAPE-PLD Blocking Peptide (aa 6-20)

NAPE-PLD Polyclonal Antibody (aa 159-172)

NAPE-PLD Polyclonal Antibody (aa 6-20)

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Downloads

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	Chemical structure definitions are available for many Cayman products. See Chemical Structure Database for details.

Batch-specific Information

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