SIRT4 (human recombinant)

Cayman Chemical Item Number 10317

Sirtuin 4; Silent Information Regulator 4; SIR2L4; NAD-dependent ADP-ribosyltransferase Sirtuin 4; SIR2-like Protein 4

Description

Source: recombinant N-terminal GST-tagged SIRT4 purified from E. coli · M_r: 61.9 kDa · There are seven human sirtuins, also known as class III HDACs, which have been designated SIRT1-SIRT7. Each is involved in various post-translational modifications by utilizing NAD dependent deacetylase and APD-ribosyltransferase activities. SIRT4 is a mitochondrial ADP-ribosyltransferase responsible for the transfer of ADP-ribose from NAD to specific substrates such as glutamate dehydrogenase (GDH).¹ In caloric-sufficient conditions, SIRT4 ADP-ribosylates GDH, repressing its activity and correspondingly decreasing insulin secretion.² There is some uncertainty about the relevance of sirtuin ribosyl transferase activity due to its very slow rate, which is about 500 times lower than the corresponding deacetylation reaction (for those that have been characterized).³ SIRT4 is found in many tissues, but is specifically enriched in the kidney, heart, brain, and liver. ²

1 Ahuja, N., Schwer, B., Carobbio, S., et al. Regulation of insulin secretion by SIRT4, a mitochondrial ADP-ribosyltransferase. J Biol Chem 282(46) 33583-33592 (2007).

2 Haigis, M.C., Mostoslavsky, R., Haigis, K.M., et al. SIRT4 inhibits glutamate dehydrogenase and opposes the effects of calorie restriction in pancreatic β cells. Cell 126 941-954 (2006).

3 Du, J., Jiang, H., and Lin, H. Investigating the ADP-ribosyltransferase activity of sirtuins with NAD analogues and 32P-NAD. Biochemistry 48 2878-2890 (2009).

Synonyms

Sirtuin 4
Silent Information Regulator 4
SIR2L4
NAD-dependent ADP-ribosyltransferase Sirtuin 4
SIR2-like Protein 4

Formulation

50 mM sodium phosphate, pH 7.2, 100 mM sodium chloride, 2.5 mM DTT, 20% glycerol

Purity

≥95%

Stability

9 months

Storage

-80°C

Shipping

Dry ice in continental US; may vary elsewhere

Background Reading

Haigis, M.C., Mostoslavsky, R., Haigis, K.M., et al. SIRT4 inhibits glutamate dehydrogenase and opposes the effects of calorie restriction in pancreatic β cells. Cell 126 941-954 (2006).

Du, J., Jiang, H., and Lin, H. Investigating the ADP-ribosyltransferase activity of sirtuins with NAD analogues and 32P-NAD. Biochemistry 48 2878-2890 (2009).

Ahuja, N., Schwer, B., Carobbio, S., et al. Regulation of insulin secretion by SIRT4, a mitochondrial ADP-ribosyltransferase. J Biol Chem 282(46) 33583-33592 (2007).

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25 µg 
$89.00

$0.00

50 µg 
$169.00

$0.00

100 µg 
$320.00

$0.00

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SIRT4 (human recombinant)